Objective To investigate the culture conditions for the establishment of cell suspension culture system for Lycoris radiata callus and its alkaloid accumulation. Methods Using the bulbs of L. radiata as explants for callus induction, calli with good quality were selected for suspension cultivation. The factors influencing the establishment of L. radiata cell suspension culture system, such as hormone, inoculum amount, inositol, and conditional culture, were investigated. The alkaloid content in cell suspens...