【摘要】 目的探讨鳖甲煎丸抗肝纤维化的作用机制。方法 SD大鼠随机分成6组:对照组,模型组,秋水仙碱(0.1 mg/kg)阳性对照组,鳖甲煎丸低、中、高剂量(0.55、1.1、2.2 g/kg)组。除对照组外,其他各组大鼠sc 40%CCl4橄榄油溶液,每周2次,连续给予6周,建立大鼠肝纤维化模型。在造模同时,各给药组每天给予相应药物10 mL/(kg·d)1次,连续给药11周。于11周末处死大鼠,免疫组化法检测肝组织中α-平滑肌肌动蛋白(α-SMA)的表达,反转录聚合酶链反应(RT-PCR)法检测大鼠肝组织中转化生长因子(TGF-β1)及smad 3基因的表达。结果与对照组比较,模型组大鼠肝组织α-SMA蛋白及TGF-β1、smad 3基因的表达显著上调(P<0.05)。与模型组比较,鳖甲煎丸和秋水仙碱可显著降低大鼠肝组织α-SMA蛋白及TGF-β1、smad 3基因的表达,其中以鳖甲煎丸2.2 g/kg的效果更显著。结论鳖甲煎丸抗肝纤维化作用机制可能与其下调TGF-β1/smad 3信号通路、抑制肝星状细胞活化和增殖有关。更多还原

【Abstract】 Objective To observe the anti-fibrotic mechanism of Biejiajian Pill.Methods SD rats were randomly divided into six groups:control,model,colchicina(0.1 mg/kg,positive),Biejiajian Pill(0.55,1.1,and 2.2 g/kg) groups.Except for the control group,the rats in the other groups were sc injected with 40% CCl4 olive oil solution twice a week for consecutive six weeks to establish the models of liver fibrosis..At the same time,drugs were ig administrated at a volume of 10 mL/kg in each group,once daily for consecutive 11 weeks.At the end of the week 11,all rats were sacrificed.The immunohistochemical technique was applied to analyzing the expression of α-smooth muscle actin(α-SMA) in liver tissue,RT-PCR was applied to analyzing the expression of transforming growth factor-β1(TGF-β1) protein and smad 3 gene in liver tissue.Results Compared with the control group,the expression levels of α-SMA,TGF-β1,and smad 3 gene increased significantly(P < 0.05) in the model group.Compared with the model group,Biejiajian Pill and colchicina decreased the expression of α-SMA,TGF-β1 and smad 3 gene,the efficacy of high-dose Biejiajian Pill group was more significantly.Conclusion The antifibrotic mechanism of Biejiajian Pill may be through lowing the TGF-β1/smad 3 signaling pathways and inhibiting hepatic stellate cells activation and proliferation.更多还原