【摘要】 目的:探讨小分子药物维生素C(VC)对CD4+效应记忆性T细胞(TEM)体外扩增的影响从而改善过继免疫治疗的效果。方法:体外分离正常人的外周血CD4+T淋巴细胞,将细胞分为2组(实验组和对照组)进行细胞培养。实验组加入VC后,通过细胞计数仪和流式细胞仪对2组细胞中TEM的扩增情况进行检测。结果:(1)在CD4+T细胞体外扩增实验中,VC对CD4+T细胞的总数无显著影响。(2)VC使TEM在CD4+T细胞中的比例显著升高,100 mg/L为扩增的最佳浓度。(3)在CD4+T细胞扩增的第10天检测其中TEM的细胞数量,对照组TEM的数量为(1.22±0.15)×106,实验组TEM的数量为(3.56±0.35)×106,两者的差异有统计学意义(P<0.01)。结论:VC可有效地促进CD4+TEM的体外扩增,为过继免疫治疗提供简单、安全及有效的体外扩增方法。更多还原

【Abstract】 AIM: To investigate the effect of vitamin C(VC),a small-molecule compound,on the expansion of CD4+effector memory T cells(TEM) in vitro and to improve the effect of adoptive immunotherapy.METHODS: Normal human peripheral blood CD4+T-lymphocytes were isolated and randomly divided into 2 groups: control group and experiment group.VC was added into experimental group.Thereafter,the expansion of CD4+TEM in the 2 groups was detected by cell counter and flow cytometry.RESULTS: VC did not significantly affect the total number of CD4+T cells,while it raised the ratio of TEM in CD4+T cells at an optimal concentration of 100 mg / L.After 10 days of the expansion of CD4+T cells,the number of CD4+TEM in experiment group was(3.56 ± 0.35) × 106,significantly larger than that in control group [(1.22 ± 0.15) × 106,P < 0.01].CONCLUSION: VC can effectively promote the expansion of CD4+ TEM in vitro,which provides a simple,safe and effective expansion method for adoptive immunotherapy.更多还原