【摘要】 目的:观察黄芪注射液对脑缺血再灌注大鼠海马组织凋亡蛋白酶激活因子1(Apaf-1)蛋白及其mRNA表达的影响。方法:将健康雄性SD大鼠120只随机分为假手术组、脑缺血再灌注组、黄芪注射液干预组和溶剂对照组。采用四血管阻断法制备大鼠脑缺血再灌注模型,除假手术组外其余3组根据再灌注不同时点再分为0 h、0.5 h、2 h、6 h、24 h、72 h和120 h 7个亚组,于再灌注相应时点提取脑组织。采用免疫组织化学和Western blot-ting检测大鼠海马组织Apaf-1蛋白的表达,RT-PCR法检测Apaf-1 mRNA的表达。结果:除0 h和120 h外,脑缺血再灌注组各个时点Apaf-1蛋白及mRNA表达均较假手术组增加(P<0.05);与脑缺血再灌注组相比,黄芪注射液干预组各个时点Apaf-1蛋白及mRNA表达均显著减少(P<0.05),而溶剂对照组各时点与脑缺血再灌注组相比则均无显著变化(P>0.05)。结论:黄芪注射液能抑制大鼠海马组织Apaf-1蛋白及mRNA表达,从而抑制脑缺血再灌注大鼠海马神经元的凋亡。更多还原

【Abstract】 AIM: To investigate the effect of Astragalus injection on the expression of apoptotic protease-activating factor 1(Apaf-1) in the hippocampus of global cerebral ische-mia-reperfusion rats.METHODS: Male SD rats were randomly divided into 4 groups with 30 each: sham operation group,cerebral ischemia-reperfusion group,cerebral ischemia-reperfusion+Astragalus injection group,and cerebral ischemia-reperfusion+vehicle group.The global cerebral ischemia-reperfusion model of the rats was established by 4-vessel occlusion.The rats in cerebral ischemia-reperfusion group,cerebral ischemia-reperfusion+Astragalus injection group and cerebral ischemia-reperfusion+vehicle group were further divided into 7 subsets,according to the reperfusion time of 0 h,0.5 h,2 h,6 h,24 h,72 h and 120 h.After reperfusion,the brains were removed at the corresponding time points.The protein expression of Apaf-1 in hippocampal neurons was detected by immunohistochemistry and Western blotting.The mRNA expression of Apaf-1 was observed by RT-PCR.RESULTS: Compared with sham operation group,the expression of Apaf-1 at mRNA and protein levels at all time points except 0 h and 120 h increased obviously in cerebral ischemia-reperfusion group(P<0.05).Compared with cerebral ischemia-reperfusion group,the expression of Apaf-1 at mRNA and protein levels at all time points except 0 h and 120 h decreased obviously in cerebral ischemia-reperfusion+Astragalus injection group(P<0.05).However,those in cerebral ischemia-reperfusion+vehicle group had no obvious change(P>0.05).CONCLUSION: Astragalus injection inhibits the expression of Apaf-1 at mRNA and protein levels in hippocampus of global cerebral ischemia-reperfusion rats,thus inhibiting the apoptosis of hippocampal neurons.更多还原