【摘要】 目的:探讨宫颈癌组织中载脂蛋白B mRNA编辑酶催化多肽样蛋白3A(APOBEC3A)表达与高危型人乳头瘤病毒(HPV)感染的关系。方法:采用免疫组化法检测26例宫颈癌、27例宫颈上皮内瘤变(CIN)I～III和22例正常宫颈组织APOBEC3A蛋白的表达,同时使用凯普分型检测试剂盒对3组样品分别进行高危HPV16/HPV18分型检测。以脂质体法转染APOBEC3A质粒进入HeLa细胞,RT-qPCR与Western blotting验证APOBEC3A对高危型HPV18 E6 mRNA以及蛋白表达的影响。结果:宫颈癌组织、CIN组织以及正常宫颈组织中APOBEC3A蛋白表达的阳性率分别为46.2%、92.6%和86.4%,宫颈癌组织中APOBEC3A蛋白表达较正常宫颈组织明显下降(P<0.01)。宫颈癌组织、CIN及正常宫颈组织中HPV16感染阳性率分别为92.3%、77.8%和54.5%;HPV18感染阳性率分别为80.8%、51.8%和68.2%;APOBEC3A蛋白表达与HPV18感染阳性率呈负相关(P<0.05)。增加HeLa细胞中APOBEC3A的表达明显降低了HPV18 E6 mRNA以及蛋白的表达。结论:在宫颈癌组织中APOBEC3A高表达可以对抗HPV18感染,并抑制HPV18 E6的转录和表达。更多还原

【Abstract】 AIM: To study the effects of human apolipoprotein B mRNA-editing catalytic polypeptide-like protein 3A(APOBEC3A) on high-risk human papillomavirus(HPV) in cervical cancer tissues.METHODS: Immunohistochemistry technique was used to detect the expression of human APOBEC3A protein,and Hybribio HPV GenoArray diagnostic kit was used to measure high-risk HPV16 and HPV18 in the specimens from 26 cases of cervical cancer,27 cases of cervical intraepithelial neoplasia(CIN) I~III and 22 cases of normal cervix.The APOBEC3A plasmid was transfected into HeLa cells by Lipofectamine.The expression of HPV18 E6 at mRNA and protein levels was examined by RT-qPCR and Western blotting.RESULTS: The protein expression of APOBEC3A in cervical cancer,CIN and normal cervix was 46.2%,92.6% and 86.4%,respectively.The protein expression of APOBEC3A in cervical cancer was significantly decreased compared with that in normal cervix(P<0.01).The infection rates of HPV16 in cervical cancer,CIN and normal cervix were 92.3%,77.8% and 54.5%,respectively,and those of HPV18 were 80.8%,51.8%,68.2%,respectively.The protein expression of APOBEC3A was negatively correlated with the infection of HPV18 in cervical tissues(P<0.05).Compared with control group,the expression of HPV18 E6 at mRNA and protein levels was decreased after APOBEC3A plasmid was transfected into HeLa cells.CONCLUSION: In cervical cancer,the high expression of APOBEC3A protein may fight against HPV18 infection,and APOBEC3A inhibits HPV18 E6 transcription and expression.更多还原