【摘要】 通过TAIL-PCR染色体步移技术,从甘蓝(Brassica oleracea L.var.capitata L.)基因组中克隆到胞质雄性不育(OguCMS)相关基因BoMF1翻译起始位点上游521bp的启动子序列。软件分析预测表明,该启动子序列中存在多个顺式作用元件,包括TATA-box、CAAT-box、MYB结合位点、植物激素响应单元等。为了研究该启动子的表达特性,亚克隆了BoMF1转录起始位点上游521bp序列,将其置换pBI121中的CaMV35S启动子,驱动其下游的GUS基因,构建植物表达载体pBI121-BoMF1P,以pBI121空载体作为阳性对照,通过农杆菌(LBA4404)介导法转入拟南芥。结果表明,甘蓝BoMF1启动子序列能驱动GUS基因在拟南芥花药发育晚期的花药和花粉中特异表达,表达具有组织特异性。更多还原

【Abstract】 The regulative sequence ( 521 bp )of OguCMS-related gene BoMF1 promoter from Brassica oleracea was cloned by genomic walking (TAIL-PCR). In silico analysis showed that this sequence contained several acting elements,including TATA-box and CAAT-box,MYB binding sites, phytohormone responsive elements and so on. In order to study the promoter function,a 521 bp promoter sequence of BoMF1 was inserted upstream of the GUS reporter gene replacing the CaMV35S promoter of pBI121. The plant expression vector pBI121-BoMF1P and pBI121 were transformed into Arabidopsis thaliana with the Agrobacterium tumefaciens strain LBA4404. It was suggested that pBI121-BoMF1P could drive the GUS gene exclusively express in anther and pollen of Arabidopsis thaliana.更多还原