【摘要】 应用杀雄菌属(Arsenophonus)23S rDNA基因和沃尔巴克氏体(Wolbachia)wsp基因的特异引物,通过PCR扩增的方法对我国20个水稻二化螟地理种群感染两类内共生菌的情况进行了检测.结果表明:我国水稻二化螟不同地理种群的Arsenophonus和Wolbachia感染率不一致,哈尔滨、惠水、吉林、南阳和扬州5个地理种群的Arsenophonus感染率为5.0%~50.0%;汉中、南宁和扬州3个地理种群的Wolbachia感染率为25.0%~40.0%,其他地理种群中没有检测到这两种共生菌的存在.水稻二化螟不同地理种群感染的Arsenophonus 23S rDNA基因序列完全一致,将该基因株型命名为csArs.但所检测到的Wolbachia wsp基因序列不一致,分别为wChisup1、wChisup5和wChisup6,其中wChisup1属于A群,其他属于B群.这说明水稻二化螟感染的Arsenophonus为同一株型,而感染的Wolbachia株型较为复杂.通过构建系统发育树发现,水稻二化螟体内的Arsenophonus 23S rDNA基因和Wolbachia wsp基因与其他物种体内相关序列完全一致或高度相似.更多还原

【Abstract】 In this study,23S rDNA from Arsenophonus and wsp gene from Wolbachia were amplified by PCR method using specific primers to study their symbiosis in Chilo suppressalis from 20 locations of China.The results indicated that Arsenophonus and Wolbachia infection rates differed widely in C.suppressalis populations.The infection rates of Arsenophonus ranged from 5.0% to 50.0% in five geographical populations from Harbin,Huishui,Jilin,Nanyang,and Yangzhou.Wolbachia ranged from 25.0% to 40.0% in three geographical populations from Hanzhong,Nanning,and Yangzhou.Their symbiosis was not observed in C.suppressalis of other locations.The sequences of23S rDNA gene which named csArs were exactly the same in the five populations.However,the sequences of wsp from the three strains of Wolbachia showed wChisup1 belonged to supergroup A,wChisup5 and wChisup6 belonged to supergroup B.The results showed the strains of Arsenophonus from C.suppressalis of the five different locations were identical,whereas the strains of Wolbachia were phylogenetically diverse.The phylogenetic analysis indicated that 23S rDNA and wsp sequences found in C.suppressalis were exactly the same with or very closely related to relevant sequences in other species.更多还原