【摘要】 目的探讨黄连与肉桂不同配伍比例对交泰丸中小檗碱溶出率的影响。方法各组中药采取水煎法,小檗碱检测条件:色谱柱XTerraP18(4.6 mm×250 mm,5μm);流动相:0.05 mol.L-1磷酸二氢钾(用磷酸调pH至3.5)-乙腈(70∶30);检测波长:346 nm;柱温:40℃;流速0.9 mL.min-1;进样量:10μL。结果黄连∶肉桂=1∶0.25,1∶0.5与黄连单煎剂小檗碱提取率差异明显,且随肉桂比例提高,小檗碱煎出率降低。结论肉桂对黄连有效成分小檗碱的溶出具一定的抑制作用。更多还原

【Abstract】 Objective To explore the effects of cinnamon and coptis in different compatibility proportion on the dissolution rate of berberine from jiaotai bolus.Methods All the drugs were decocted and berberine content was determined on HPLC with a UV detector based on the following parameters: XTerraP18(4.6 mm×250 mm,5 μm) column,acetonitrile-0.05 mmol·L-1 potassium dihydrogenphosphate(30:70,pH 3.5 adjusted by phosphoric acid) as mobile phase,detection wavelength 346 nm,column temperature 40 ℃,flow rate 0.9 mL·min-1 and loading sample volume 10 μL.Results There was marked difference in the extraction ratio of berberine between cinnamon combined with coptis in the proportion of 1:0.25,1:0.5,and coptis single decoction.Moreover,the decoction rate of berberine decreased with cinnamon proportion increasing.Conclusion Cinnamon has inhibitory effect on dissolution of berberine from coptis.更多还原