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氧化低密度脂蛋白诱导小鼠心脏内皮细胞炎性反应中Toll样受体2的表达

The expression of TLR2 receptor in cardiac endothelial cells isolated from mice with inflammatory response induced by ox-LDL

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【作者】 李吉林侯夏凯薛爱玲王欣许端敏王伟

【Author】 LI Jilin;HOU Xiakai;XUE Ailing;WANG Xin;XU Duanmin;WANG Wei;Department of Cardiology, the Second Affiliated Hospital of Shantou University Medical College;Medical College of Shantou University;Department of Cardiology, the First Affiliated Hospital of Shantou University Medical College;

【机构】 汕头大学医学院第一附属医院心血管内科汕头大学医学院汕头大学医学院第二附属医院心血管内科

【摘要】 目的研究氧化低密度脂蛋白(ox-LDL)诱导小鼠心脏内皮细胞炎性反应中Toll样受体(TLR)2的表达。方法分离小鼠心脏内皮细胞,对照组给予DMEM培养,干预组分别给予不同浓度的ox-LDL(20,40μg/mL)作用细胞24 h,采用Western blot法检测细胞间黏附分子-1(ICAM-1)、TLR2的表达,利用ELISA方法检测炎症因子白细胞介素(IL)-6、IL-8的变化。结果与对照组相比,ox-LDL干预组TLR2蛋白表达减少(P<0.01),ICAM-1蛋白表达增多(P<0.01),IL-6和IL-8表达明显增加(P<0.05);与低浓度组比较,高低浓度组ICAM-1、IL-6和IL-8表达差异无统计学意义(P>0.05)。结论 ox-LDL可以诱导小鼠心脏内皮细胞的炎性反应,同时可能抑制了TLR2受体的表达。

【Abstract】 Objective To research the expression of TLR2 receptor in cardiac endothelial cells isolated from mice with inflammatory response induced by ox-LDL. Methods Cardiac endothelial cells were isolated from mice, ox-LDL(20, 40 μg/mL) was added into the medium for 24 h retrospectively in the treatment group, while only DMEM was added in the control group. Chemokine ICAM-1 and TLR2 receptor was detected by Western blot, cytokine IL-6 and IL-8 were determined by ELISA. Results Compared with the control group, ICAM-1, IL-6 and IL-8 increased in the ox-LDL treatment group(P < 0.05 or P < 0.01), while TLR2 receptor decreased in the ox-LDL treatment group(P < 0.01). There were no differences between low and high concentration group in the expression of ICAM-1, IL-6 and IL-8(P > 0.05). Conclusion ox-LDL can induce inflammatory response in cardiac endothelial cells isolated from mice, while may inhibit the expression of TLR2 in cells.

【基金】 广东省医学科研基金项目(编号B2012249);广东省大学生创新创业训练计划项目(编号1056012085)
  • 【文献出处】 中国医药导报 ,China Medical Herald , 编辑部邮箱 ,2013年35期
  • 【分类号】R363
  • 【被引频次】1
  • 【下载频次】96
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