【摘要】 目的探讨金属蛋白酶9(MMP9)、表皮生长因子受体(EGFR)、血管内皮生长因子(VEGF)在非小细胞肺癌(NSCLC)中的表达及与预后的关系。方法选取术后经病理证实的40例NSCLC标本为NSCLC组,10例肺良性病变为对照组。采用免疫组化MAXvision法检测MMP9、EGFR、VEGF在两组中的表达,采用χ2检验分析MMP9、EGFR、VEGF在NSCLC及肺良性病变中的表达差异,用Kaplan-Meier生存曲线探讨MMP9、EGFR、VEGF在NSCLC组织中的表达及其与预后的关系,采用Cox多因素回归分析法分析影响远期疗效的相关因素。结果 MMP9、EGFR、VEGF在NSCLC组中的表达水平明显高于对照组(P<0.05)。MMP9、EGFR、VEGF蛋白阴性表达组3年生存率明显高于阳性表达组(P<0.05),阳性表达率越高,预后越差。影响NSCLC远期疗效的相关因素有年龄、吸烟史、病程、病理分期、淋巴结转移(P<0.05)。结论 MMP9、EGFR与VEGF参与NSCLC的发生和发展,阳性表达的NSCLC患者预后差。NSCLC的远期疗效与患者的年龄、吸烟史、病程、病理分期、淋巴结转移呈正相关。更多还原

【Abstract】 Objective To observe the expression of matrix metalloproteinase 9(MMP9),epidermal growth factor receptor(EFGR) and vascular endothelial growth factor(VEGF) and its relationship with prognosis in the non-small cell lung cancer(NSCLC).Methods 40 cases of NSCLC samples which were confirmed by pathology were selected as NSCLC group,and 10 samples with lung benign lesion were selected as control.The expression levels of MMP9,EGFR and VEGF were detected by immunohistochemistry MAXvision method,and the differences between the two groups were analyzed by square test.The expressions of MMP9,EGFR and VEGF in NSCLC and its relationship with prognosis were analyzed by survival curve of Kaplan-Meier.The relative factors of long-term effect were analyzed by multiple regression analysis of Cox.Results The expression levels of MMP9,EGFR and VEGF in observation group were significantly higher than those in the control group(P < 0.05).3 years survival rate of patients with negative production of MMP9,EGFR and VEGF protein was significantly higher than patients with positive production of MMP9,EGFR and VEGF protein(P < 0.05).The higher the positive expression rate,the worse prognosis.Age,history of smoking,course of disease,pathological stage,and lymphatic metastasis were relevant factors of the long-term therapeutic of NSCLC(P < 0.05).Conclusion MMP9,EGFR and VEGF participate in the occurrence and development of NSCLC,and positive expression are associated with poor prognosis of NSCLC patients.Age,history of smoking,course of disease,pathological stage,and lymphatic metastasis are associated with the long-term therapeutic effect of NSCLC.更多还原