【摘要】 目的分析牡蛎过敏原Cra g 1的二级结构及其B、T细胞表位,为探索基于抗原表位的免疫治疗奠定基础。方法从Uniprot数据库中获得牡蛎过敏原Cra g 1的氨基酸序列,通过DNA Star Protean软件,采用Garnier-Robson方法和Chou-Fasman方法分析其二级结构;采用Kyte-Doolittle法、Karplus-Schulz法、Emini法、Jameson-Wolf法综合分析Cra g 1主要的B细胞抗原表位。使用SYFPEITHI和NetMHC-Ⅱ在线网站分析T细胞抗原表位。结果牡蛎过敏原Cra g 1的二级结构主要为α-螺旋,其B细胞表位接近覆盖Cra g 1氨基酸全序列;而T细胞优势表位位于第89~103、108~122、129~143位肽段。结论本研究分析牡蛎过敏原Cra g 1的B、T细胞优势表位,为日后Cra g 1的基础免疫学研究,如疫苗的研制、诊断试剂的制备和其抗原性改造等提供了理论依据。更多还原

【Abstract】 Objective To analyze the secondary structure and B,T cell epitopes of the oyster major allergen Cra g 1,develop the immunotherapy of oyster allergy based on antigenic epitopes.Methods The amino acid sequence of oyster major allergen Cra g 1 was acquired from Uniprot database.The secondary structure of Cra g 1 was analyzed by DNAStar-Protean software with Garnier-Robson method and Chou-Fasman method.The B cell epitopes of Cra g 1 were analyzed synthetically by Kyte-Doolittle method,Karplus-Schulz method,Emini method and Jameson-Wolf method.The T cell epitopes of Cra g 1 were analyzed by SYFPEITHI and NetMHC-Ⅱ online website.Results The analysis of secondary structure showed Cra g 1 was mainly constituted by α-spiral.B cell epitopes covered almost the whole amino acid sequence of Cra g 1.However,T cell epitopes analysis demonstrated that the regions of 89-103,108-122 and 129-143 were the dominant T cell epitopes.Conclusion This study,which analyzed the dominant B,T cell epitopes of oyster allergen Cra g 1,provides a theoretical basis for further basic immunology researches of Cra g 1,such as vaccine development,diagnosis reagents preparation,and its antigenicity modification.更多还原