【摘要】 目的研究亚麻醉剂量氯胺酮对神经痛(NP)大鼠模型的镇痛作用及对海马NR2B亚单位的影响。方法采用坐骨神经慢性压迫法(CCI)制备大鼠NP模型。大鼠随机分为4组(n=6):假手术组(SO组)、模型组(NP组)、K1、K2组。K1和K2组于CCI术后7～31 d每天分别腹腔注射氯胺酮10、20 mg/kg。各组分别于术前1 d和术后3、7、21、31 d测定大鼠术侧后爪痛阈;术后第31天,用RT-PCR和Western blot的方法检测大鼠海马组织中NR2B mRNA和蛋白的表达水平。结果与SO组比较,NP、K1组和K2组术后痛阈明显降低(P<0.05),手术对侧海马组织中NR2B表达水平增高(P<0.05)。与NP组比较,K1组和K2组术后痛阈升高(P<0.05),海马的NR2B蛋白表达降低(P<0.05)。结论亚麻醉剂量氯胺酮可以部分反转NP模型痛觉过敏和海马的NR2B亚单位表达增高。更多还原

【Abstract】 Objective To evaluate the analgesic effect of Ketamine on a rat model of neuropathic pain(NP) and expres sion of NR2B subtype in hippocampus.Methods NP model was prepared by chronic constrictive injury(CCI) of sciatic nerve.Rats were randomly divided into four groups(n = 6): Sham operation group(SO group),NP group,K1 group,K2 group.K1 and K2 group were daily intraperitoneally injected ketamine at subanesthetic dose of 10 mg/kg and 20 mg/kg body weight respectively for 7-31 days after CCI operation.Pain thresholds of hind legs of NP rats were mea sured on the preoperative 1 day and 3,7,21,31 days after operation respectively.On the 31st day,NR2B mRNA and protein expression level in hippocampus was estimated by using RT-PCR and Western blot.Results Compared with SO group,the pain thresholds were reduced significantly in NP,K1,K2 group(P < 0.05);the NR2B expression levels in the contralateral hippocampus were increased(P < 0.05) in NP,K1 and K2 groups.Compared with NP group,the pain thresholds were increased in K1,K2 group(P < 0.05),the NR2B expression reduced(P < 0.05).Conclusion Subanesthetic doses of ketamine may reverse partially hyperalgesia and down regulate the high expression of NR2B in hippocampus of NP model.更多还原