【摘要】 目的探讨硫化氢在急性非结石性胆囊炎中的作用。方法 40只豚鼠随机分为4组:假手术组、胆总管结扎48h组、胆总管结扎48h+NaHS组、胆总管结扎48h+DL-炔丙基甘氨酸(PAG)组。除假手术组外,其余3组均游离胆总管,在胆道进入十二指肠入口处结扎胆总管。胆总管结扎48h+NaHS组和胆总管结扎48h+PAG组分别于术前30min腹腔注射NaHS10mg/kg、PAG 50mg/kg。待48h后取各组血清及胆汁,测定其中H2S含量,取胆囊组织,行HE染色、MPO免疫组化。结果假手术组胆囊未见明显炎症改变,胆总管结扎48h组炎症改变明显,但较胆总管结扎48h+PAG组轻,较胆总管结扎48h+NaHS组重。血浆H2S含量为:胆总管结扎48h+NaHS组>胆总管结扎48h组>胆总管结扎48h+PAG组>假手术组(各组间比较,P<0.05)。胆汁H2S含量为:胆总管结扎48h+PAG组>胆总管结扎48h组>胆总管结扎48h+NaHS组>假手术组(各组间比较,P<0.05)。结论利用胆总管结扎的方法可以构建AAC的模型。H2S对AAC的炎症起抑制作用。胆汁中H2S含量的变化,可能与产H2S的细菌感染有关。更多还原

【Abstract】 Objective To investigate the effects of hydrogen sulfide on acute acalculous cholecystitis. Methods Guinea pigs were divided into 4 groups randomly: sham group,CBDL group,CBDL + NaHS group,CBDL + DL- propargylglycine( PAG) group. Except of the sham group,the rest three groups were freed and ligated common bile duct at the entrance of bile duct into the duodenum. The CBDL + NaHS group and CBDL + PAG group were given NaHS 10mg/kg and PAG 50mg/kg respectively by intraperitoneal injection before 30minutes of the operation. 48 hours later,the serum and bile were taken out of every group and the content of H 2 S in them was determined,HE stain and immunohistochemistry of MPO was done for the gallbladders. Results The sham group had not obvious inflammatory change,while CBDL- 48h group had. But the inflammatory change in CBDL- 48h group was lighter than that in CBDL- PAG group, heavier than that in CBDL- NaHS group. H 2 S content in plasma was that of CBDL- 48h + NaHS group > CBDL- 48h group > CBDL- 48h + PAG group > sham group( each group,P < 0. 05). H 2 S content in bile was that of CBDL- 48h + PAG group > CBDL- 48h group > CBDL- 48h + NaHS group > sham group( each group,P < 0. 05). Conclusion Using the method of common bile duct ligation can build the model of AAC. H 2 S has inhibitory effect on the inflammation of AAC. The changes of H 2 S content in bile may be related to the infection of bacterials producing H 2 S.更多还原