【摘要】 目的探讨高压氧(HBO)治疗后立即行放射性药物32P胶体治疗对大鼠种植瘤生长及血管内皮生长因子(VEGF)表达的影响。方法完成大鼠乳腺癌Walker-256细胞种植性肿瘤模型的制作1周后,将80只实验动物随机分为A、B、C、D4组,即对照组、高压氧治疗组(HBO组)、32P胶体治疗组、胶体32P后高压氧治疗组(联合治疗组),每组20只。A、C组通过瘤体内直接注射法分别给予相同体积的生理盐水、32P胶体;B组注入生理盐水后连续高压氧治疗14天;D组注射32P胶体后行高压氧治疗持续14天。测定各组肿瘤7、12、20天时的体积,并计算生长率;通过免疫组化技术检测肿瘤组织的血管内皮生长因子(VEGF)的表达。结果 A、B、C、D组第12、20天时的肿瘤生长率分别为232.12%±67.52%、878.64%±425.36%;208.23%±78.78%、845.02%±632.67%;145.65%±55.37%、502.06%±312.96%;104.18%±43.75%、113.86%±42.38%,联合治疗组明显小于其他各组(P<0.05)。A、B、C、D组肿瘤组织中VEGF阳性表达率分别为90.0%(18/20)、80.0%(16/20)、30.0%(6/20)、10.0%(2/20),A、B组之间无统计学差异(P>0.05),A、B组的阳性表达率明显高于C、D组(P<0.05),A组尤为明显,C、D组之间存在明显差异(P<0.05)。结论高压氧联合放射性药物32P胶体可显著抑制大鼠种植瘤的生长,其机制可能与抑制VEGF的表达、肿瘤血管新生,从而抑制癌细胞增殖有关。更多还原

【Abstract】 Objective To investigate the effect of 32P- colloid therapy immediately after hyperbaric oxygen treatment on implanted tumor growth and serum VEGF expression in rats.Methods Immediate Walker- 256 cancerous ascetic injection was used to make a rat tumor- bearing model,then the 1w later 80 rats were randomly divived into control group(A group),hyper- baric oxygen group(B group),32P- colloid group(C group),cooper- ration of hyperbaric oxygen and32P- colloid group(D group),with 20 in each group.Ratsin A and C group by the direct injection of tumors in vivo were respectively given the same volume of saline and32P- colloid.Ratsin B group were treated with hyperbaric oxygen therapy for 14 days after the injection of saline.Ratsin D group were given hyperbaric oxygen therapy for 14 days after the injectin of32P- colloid.Ratsin Every group were measured tumor volume on 7th,12th and 20th day to calculate its growth rate.The vascular endothelial growth factor(VEGF) was performed by immunohistochemistry method.Results The tumor growth rates of the 12th and 20th day in A,B,C and D groups were 232.12% ± 67.52% and 878.64% ± 425.36%,208.23% ± 78.78% and 845.02% ± 632.67%,145.65% ± 55.37% and 502.06% ± 312.96%,104.18% ± 43.75% and 113.86% ± 42.38% respeltirely.The rate of D group was significantly less than those of A,B and C groups(P < 0.05).The rate of C group was obviously less than those of A and B groups(P < 0.05).There was no distinguished difference between the rates of A and B groups(P > 0.05).The VEGF positive expression rates in A,B,C and D groups were 90.0%(18 /20),80.0%(16 /20),30.0%(6 /20),10.0%(2 /20).There was no significant difference between A and B groups(P > 0.05).The positive expression of A and B groups was significantly higher than that of C and D groups(P < 0.05).There was distinguished difference between C and D groups(P < 0.05).Conclusion The tumor growth was inhibited significantly after the treatment of hyperbaric oxygen in combination with32P- colloid.The mechanism may being the expression and the angiogenesis of blood vessel being inhibited,then the proliferation of cancer cells being inhibited.更多还原