【摘要】 目的体外复制细胞炎性损伤模型用于体外炎症研究。方法将人脐静脉内皮细胞(HUVEC)培养后分组,用不同浓度(0、0.1、1、10、20μg/ml)的脂多糖(LPS)刺激,在不同时间点(4、12、24、48h)收样品,检测细胞上清中的白介素-6(IL-6)水平。结果每个时间点的HUVEC:LPS(0.1、1、10、20μg/ml)比LPS(0μg/ml)刺激后IL-6分泌明显增高(P<0.05);48h内随时间增加,IL-6分泌增加,呈线性关系;相同时间点不同LPS浓度(0.1、1、10、20μg/ml)刺激细胞产生的IL-6分泌差异无统计学意义(P>0.05)。结论体外LPS刺激HUVEC是理想的细胞炎性损伤模型。更多还原

【Abstract】 Objective To construct cell inflammatory injury model in vitro for inflammation research.Methods Human umbilical vein endothelial cells(HUVEC)were cultured and gronged,cells then were stimulated with different concentrations of lipopolysaccharide(LPS).cells were Collected at different time points(4,12,24,48h)and interleukin-6(IL-6) levels of cell supernatant were detected.Results HUVEC in any time points:IL-6 secretion of different concentration of LPS(0.1,1,10,20μg/ml) was significantly higher than the negative control(0μg/ml)(P<0.05).The secretion of IL-6 increased linearly with time in 48h.No statistically significant differences in secretion of IL-6 stimulated by different concentration of LPS(0.1,1,10 and 20μg/ml) at the same time(P>0.05).Conclusion HUVEC stimulated by LPS is a cell inflammatory injury model available in vitro.更多还原