【摘要】 目的探讨TRPV1受体激动剂辣椒素在脓毒症肺损伤保护机制中的作用。方法选取ICR雄性小鼠共108只,随机分成6组(各18只):正常对照组(CON组)、辣椒素对照组(CAP+CON组)、辣椒素受体拮抗剂对照组(CAPZ+CON组)、脓毒症组(LPS组)、辣椒素+脓毒症组(CAP组)、辣椒素受体拮抗剂+脓毒症组(CAPZ组)。LPS组、CAP组、CAPZ组均腹腔注射LPS 10mg/kg以制备脓毒症急性肺损伤模型,CON组、CAP+CON组、CAPZ+CON组经腹腔注射等量生理盐水。在造模前0.5hCAP组、CAP+CON组等两组小鼠预先腹腔注射辣椒素,CAPZ组、CAPZ+CON组等两组小鼠预先腹腔注射辣椒素受体拮抗剂。每组取注射后3、8、16h等各点检测血清TNF-α、IL-6变化(6只),并测定肺组织湿/干重(W/D)比值和评价其病理学改变,进行统计分析。结果与CON组比较,LPS组、CAP组、CAPZ组血清IL-6和TNF-α在3、8、16h各点上升。与LPS组比较,CAP组IL-6和TNF-α在3、8、16h各点下降。与LPS组比较,CAPZ组IL-6在3、8、16h各点上升,TNF-α在3、8h各点上升。与CON组比较,LPS组、CAP组、CAPZ组肺组织W/D值在8、16h时升高。与LPS组比较,CAP组肺组织W/D在8、16h时降低。与LPS组比较,CAP组肺病理改变程度减弱,CAPZ组病理改变不明显。结论 TRPV1受体抑制可能是脓毒症肺损伤的机制之一,激活TRPV1受体能减轻脓毒症肺损伤。更多还原

【Abstract】 Objective To investigate the effect of TRPV1 receptor agonist,capsaicine for LPS-induced acute lung injury in mice.Methods Totally 108 ICR male mice were randomly divided into six groups(n=18 for each):control group(CON group),capsaicine+control group(CAP+CON group),capsazepine+control group(CAPZ+CON group),Lipopolysaccharide group(LPS group),capsaicine group(CAP group) and capsazepine group(CAPZ group).Mice in LPS group,CAP group and CAPZ group were injected intraperitoneally with LPS(10mg/kg) to induce acute lung injury.Capsaicine in CAP group and CAP+CON group while capsazepine in CAPZ group and CAPZ+CON group were injected intraperitoneally 0.5h before LPS,respectively.Values of serum TNF-α,IL-6,pathomorphologic changes of lung,W/D ratio were detected after 3,8,16h.Results Compared with CON group,values of TNF-α,IL-6 in LPS group,CAP group and CAPZ group were upregulated at 3h,8h and 16h.Compared with LPS group,values of TNF-α and IL-6 in CAP group were decreased at 3,8 and 16h while values of IL-6 were upregulated at 3,8 and 16h and values of TNF-α were upregulated at 3 and 8h in CAPZ group.Compared with CON group,W/D ratio were upregulated in LPS group,CAP group and CAPZ group.Compared with LPS group,W/D ratio of lung tissues were decreased in CAP group at 8 and 16h.The pathological changes in lung tissue of CAP group were milder than those in LPS group.Conclusion Pretreated with capsaicine(TRPV1 receptor agonist) can improve the lung injury of sepsis while pretreated with Capsazepine(TRPV1 receptor antagonist) maybe worsen it.So we think blocking TRPV1 receptor is a reason for lung injury of sepsis and activating TRPV1 receptor may be a protective factor for it.更多还原