【摘要】 目的探讨慢性乙肝病毒(HBV)感染患者外周血中树突状细胞(DC)亚群比例及其临床意义。方法选取2011年3～11月笔者医院慢性HBV感染患者29例,分为HBeAg(-)组13例、HBeAg(+)16例,以18名健康体检者作为对照。流式细胞术检测髓样DC(mDC)和浆细胞样DC(pDC)比例,实时荧光定量PCR检测HBV-DNA。结果对照组、HBeAg(-)组、HBeAg(+)组中pDC比例分别为0.35%(0.18%～0.37%)、0.19%(0.10%～0.25%)、0.06%(0.02%～0.08%),差异两两之间均具有统计学意义(P<0.05)。HBV感染者的HBV-DNA复制数的常用对数和外周血pDC存在显著负相关(r2=0.448,P<0.01)。结论慢性HBV感染患者患者外周血pDC的减少可能和病毒载量及HBeAg转换有关。更多还原

【Abstract】 Objective To investigate the clinical significance of dendritic cells subsets detection in peripheral blood of patients with HBV chronic infection.Methods 29 patients with HBV chronic infection from March 2011 to November 2011 were selected,including 13 patients with HBeAg-negtive and 16 patients with HBeAg-positive.18 healthy people were set as the normal control.The rates of myeloid DC(mDC) and plasmacytoid DC(pDC) were detected by flow cytometry.The HBV-DNA loads were detected by real-time quantitative polymerase chain reaction(RT-PCR).Results The rates of pDC in control group 0.35%(0.18%-0.37%),HBeAg-Negtive group 0.19%(0.10%-0.25%) and HBeAg-positive group 0.06%(0.02%-0.08%) were significantly different from each other(P<0.05).There was a significant negative correlation between lg of HBV-DNA copies and rates of pDC(r2=0.448,P<0.01).Conclusion The reduction of pDC rates in patients with HBV chronic infection is associated with HBV-DNA load and HBeAg conversion.更多还原