【摘要】 目的利用EB病毒转化人外周血B淋巴细胞,建立浙江地区原发性免疫缺陷病患儿永生化B淋巴细胞系。方法利用B95-8细胞常规制备EB病毒上清,用环孢菌素A法转化外周血B淋巴细胞。结果成功建立15例原发性免疫缺陷病患儿永生化B淋巴细胞系,转化后细胞增殖活跃。结论 EB病毒加环孢菌素A能成功转化外周血B淋巴细胞为永生化B淋巴细胞系,为进一步研究原发性免疫缺陷病的分子遗传机制和细胞遗传机制提供了实验材料。更多还原

【Abstract】 Objective To establish Epstein-barr virus transformed B lymphoblastic cell lines(BLCLs) from primary immunodeficiency patients in vitro in Zhejiang province.Methods We used routine method accept Epstein-barr virus and cyclosporine method to transform human B lymphocyte into immortal lymphoblast cell lines.Results We successfully established 15 immortalized lymphoblast cell lines,and they proliferated actively.Conclusion Epstein-barr virus plus cyclosporine A successfully transformed peripheral blood B lymphocytes into immortalized B lymphocytes and BLCLs provide experimental material for studying molecular genetic mechanism and cell genetic mechanism in primary immunodeficiency diseases in future.更多还原