【摘要】 目的观察共刺激分子(costimulatory molecule)OX40在急性肝衰竭(acute liver failure,ALF)大鼠肝组织中表达的变化,探讨OX40在急性肝衰竭发病机制中的作用。方法雄性SD大鼠,随机分为正常对照组、急性肝衰竭(ALF)模型组。急性肝衰竭组:腹腔同时注射D-氨基半乳糖(D-GalN)800mg/kg和脂多糖(LPS)8微克/只,在D-GalN和LPS注射后6、12、24、48h 4个时间点留取大鼠血及肝脏标本。全自动生化仪检测血清ALT、AST水平。苏木素-伊红(HE)染色下观察肝组织变化。RT-PCR法检测大鼠肝组织OX40mRNA表达。Western blot法检测肝组织OX40蛋白表达。结果肝衰竭组血清ALT和AST水平在12h升高最明显,24h开始下降。肝衰竭组肝组织OX40mRNA水平至12h达峰值,之后逐渐下降,与对照组比较,各时间点差异具有统计学意义(F=29.970,162.975,62.476,25.124,P<0.05)。肝衰竭组OX40蛋白的表达于12h达到最大值,与对照组相比,差异有统计学意义(F=17.240,169.298,88.289,74.984,P<0.05)。结论 OX40在急性肝衰竭过程中呈上升趋势,于12h达高峰,提示OX40可能在急性肝衰竭过程中发挥重要作用。更多还原

【Abstract】 Objective To observe the expression ofcostimulatory molecule OX40mRNA in liver tissues ofrats with acute liverfailure(ALF),and discusses for the mechanisms of OX40 in ALF.Methods The male SD rats were divided into two groups randomly.ALFmodel of SD rats was injected with D-GalN 800mg/kg and LPS 8μg/rat.Liver tissue and blood were collected on 6,12,24,48 hours after the injection of D-GalN/ LPS.Liver histopathology and fine structure of each group were observed by HE staining and electron microscope.OX40mRNA of liver tissues were determined by RT-PCR and Western blot was used to detect protein expressions of OX40.Results The level of ALT and AST in model group peaked at 12 hours,decreased at 24 hours.The level of OX40 peaked at 12 hours and decreased gradually.Compared with the normal group,there was significant different at different time points(F=29.970,162.975,62.476,25.124,P<0.05).The tendency of OX40 protein expression in the model group peaked at 12 hours and the discrepancy between model group and normal group had statistical significance(F=17.240,169.298,88.289,74.984,P<0.05).Conclusion The level of OX40 in ALF group tended to increase and peaked at 12 hours,which suggested that OX40 played an important role in the process of acute liver failure.更多还原