【摘要】 目的探讨间皮组织细胞极性修复对癌细胞生长抑制的影响。方法分别采用细胞极性修复实验、消化道吻合口愈合影响实验、"T"管窦道形成实验、细胞极性修复相关指标检测、永生化细胞(人膀胱癌细胞株T24)抑制实验等一系列从动物实验到细胞实验,采用SurgetakeTM或生理盐水进行了对照研究。结果兔实验结果显示,实验组10例均实现极性修复,而对照组均无完全实现极性修改;同时实验表明SurgetakeTM具有较强的激活体内t-PA的活性,用药后实验组t-PA达到(1.6±0.8)IU/ml,而对照组t-PA为(0.9±0.4)IU/ml,两组比较差异有统计学意义(P<0.01)。永生化细胞(人膀胱癌细胞株T24)抑制实验实验结果显示surgetakeTM组3个视野计数细胞总数为844个,对照组3个视野细胞总数为2295个。结论 SurgetakeTM具有保持间皮组织极性的作用,实现了间皮结构的重建,能使t-PA增加,细胞极性增强,组织修复的就好。细胞的极性保持过程与小分子蛋白t-PA相关,这一过程的极化作用可使肿瘤细胞恶性程度降低。更多还原

【Abstract】 Objective To determine the effects of repair of mesothelium polarity on cancer cell inhibition.Methods Serial animal and cytological experiments,including cell polarity repair test,alimentary tract anastamosis healing test,T-tube fistula formation test,assessment of the parameters associated with cellular polarity repair and immortal cell(human bladder cancer cell line T24) inhibition,were employed in the control trials by using SurgetakeTM and normal saline.Results The experiments on rabbits evidenced a markedly higher proportion of complete repair of cellular polarity in SurgetakeTM group(n=10) than that in control group(P<0.01).SurgetakeTM more potently potentiated the t-PA activity in vivo [(1.6±0.8)IU/ml vs(0.9±0.4)IU/ml],P<0.01) and yielded a higher cell count in three visual fields when compared with normal controls(2995 vs 844,P<0.05),as evidenced by immortalized cell inhibition test.Conclusion SurgetakeTM achieves mesothelium reconstruction by retaining tissue polarity and benefits the tissue repair from elevated t-PA activity leading to potentiated cellular polarity.The fact that maintenance of polarity correlate considerably with t-PA,a low molecular weight protein,indicates reduced malignancy via cellular polarization.更多还原