【摘要】 目的:建立测定四氢异喹啉(Sal)和五羟色胺(5-HT)的电分析化学新方法。方法:首先通过电化学方法制备了铂纳米/碳纳米管复合材料(Pt NPs/MWCNTs)修饰的玻碳电极,并通过扫描电镜研究了复合材料形貌,同时也研究了Sal和5-HT在Pt NPs/MWCNTs修饰电极上的电化学行为。结果:Sal和5-HT的电化学氧化信号有着明显的分离,对于Sal,峰电流与4.8×10-7~5.2×10-5mol·L-1呈线性变化(r=0.9995),检测限为1.6×10-7mol·L-1(S/N=3);而对于5-HT,峰电流与5-HT在2.7×10-7~3.07×10-5mol·L-1范围内呈线性增加(r=0.9993),检测限为9.0×10-8mol·L-1(S/N=3)。结论:本方法可以应用于血样中Sal和5-HT的同时灵敏检测,结果令人满意。更多还原

【Abstract】 Objective: To establish a new electroanalytical method for simultaneous determination of salsolinol( Sal) and 5- hydroxytryptamine( 5- HT) in plasma samples. Methods: Pt NPs/MWCNTs composite material was prepared,and its morphology was obtained by scanning electron microscopy. The electrochemical behavior of Sal and 5- HT was investigated at Pt NPs / MWCNTs modified electrode. Results: The experiment result showed the oxidation signals of 5- HT and Sal were obviously separated,and the intensity of the peak current for Sal was linearly related to the concentration in range from 4. 8 × 10- 7to 5. 2 × 10- 5mol·L- 1with a detection limit of 1. 6 × 10- 7mol ·L- 1( S/N = 3). The intensity of the peak current for 5- HT was linearly related to the concentration in range from 2. 7 × 10- 7to 3. 07 × 10- 5mol·L- 1with a detection limit of 9. 0 × 10- 8mol·L- 1( S/N =3). Conclusion: The modified electrode can be used for simultaneous sensitive detection of Sal and 5- HT in serum samples and it has obtained satisfactory results.更多还原