【摘要】 目的:探讨不同加工方法对泸州山银花品质的影响,为泸州山银花加工方法的筛选提供依据。方法:采用阴干、晒干、真空干燥、远红外干燥、微波干燥、烘干6种方法加工山银花,对外观性状进行描述,采用高效液相色谱法测定山银花中新绿原酸、绿原酸、异绿原酸A、异绿原酸C的含量。色谱柱为Kromasil C18柱(250 mm×4.6 mm,5μm);流动相为乙腈-0.1%磷酸水溶液,梯度洗脱;检测波长326 nm;柱温30℃;流速1 mL·min-1;进样量10μL。结果:6种加工方法中,真空干燥样品中新绿原酸、绿原酸、异绿原酸A及异绿原酸C的含量分别为0.094%、2.982%、1.804%、0.255%,高于其他5种方法所得样品;外观颜色和油润度均比其他5种方法好。结论:真空干燥方法得到的样品外观品相好,测得的新绿原酸、绿原酸、异绿原酸A及异绿原酸C含量高,明显优于其他干燥方法。更多还原

【Abstract】 Objective: To discuss the effects of different drying methods on officinal qualities of Flos Lonicerae.Methods: The following six methods were used: drying in the shade,drying in the sun,vacuum drying,far infrared drying,microwave drying,and oven drying. External qualities were studied and the contents of neochlorogenic acid,chlorogenic acid,isochlorogenic acid A and isochlorogenic acid C were determined by HPLC. HPLC was performed on a Kromasile C18column( 250 mm × 4. 6 mm,5 μm) at 30℃. The composition of mobile phase was acetonitrile-0. 1% phosphoric acid with gradient elution,the flow rate was 1 mL·min-1,and the injection volume was 10 μL.Results: The content of neochlorogenic acid,chlorogenic acid,isochlorogenic acid A and isochlorogenic acid C in the vacuum drying sample was 0. 094%,2. 982%,1. 804% and 0. 255% respectively,which was obviously higher than samples dried by other five methods; the color and texture of the vacuum drying sample was also better.Conclusion: The appearance and contents of chlorogenic acids of the vacuum drying sample are better than those of the others,indicating that the vacuum drying method is obviously better than the others.更多还原