【摘要】 WRKY基因家族是主要存在于植物中的转录因子,拟南芥中至少有 74 个成员。根据锌指结构特征和 WRKY 结构域的数目,可以将 WRKY 转录因子分为三大类。拟南芥 WRKY68 属于第Ⅱ类 WRKY 蛋白。通过GUS染色和qRT-PCR 分析各组织部位的表达情况,发现WRKY68在根中的表达量是最高的,其次是幼嫩的叶片和老的荚果中。各种处理条件下的表达水平显示,IAA 和高温处理后,WRKY68 的表达明显上调,PstDC3000、JA、SA、NAA 轻微诱导WRKY68的表达,而 Botrytis、NaCl、甘露醇、PEG、脱水、ACC、ABA 抑制 WRKY68 的表达,根据以上实验结果,我们推测 WRKY68 可能参与生长素和温度调控的植物形态建成及发育过程。更多还原

【Abstract】 The transcription factors WRKY protein superfamily are mainly presented in plants,which contains at least 74 members in Arabidopsis. It can be classified into three groups according to the number of WRKY domains and the features of their zinc-finger-like motif. Arabidopsis WRKY68 is one of the group Ⅱ WRKY proteins. GUS staining and qRT-PCR analysis showed that the expression of WRKY68 in roots was the highest in all tissues and the second was in old siliques and young leaves. The expression profiles indicated that the expression levels of WRKY68 were elevated by IAA and heat treatments,and slightly induced by PstDC3000, JA, SA, NAA while which were inhibited by other treatments including Botrytis, NaCl, mannitol, PEG, dehydration, ACC, ABA.These results suggested that WRKY68 might be involved in plant morphogenesis and development which is regulated by auxin and temperature.更多还原