A HPLC method for the simultaneous separation and determination of gibberellin A3(GA),indoleacetic acid(IAA) and 6-furfurylaminopurine(6-KT) was developed after the optimizing of the chromatographical conditions like the composition and concentration of the mobile phase together with the flow rate and column temperature.Gibberellin A3,indoleacetic acid and 6-furfurylaminopurine in the microbe and its fermentation broth could be separated and determined effectively within 12 min by using Waters C18(4.6 mm×25...