【摘要】 目的:观察人组织激肽释放酶(HTK)对大鼠坐骨神经损伤的修复效应。方法:选取36只雄性SD大鼠,重量在180～220g之间,分离坐骨神经,造成坐骨神经挤压伤模型,然后随机均分成3组:对照组,自尾静脉每日注射生理盐水2mL;甲强龙组(SM组),自尾静脉每日注射甲强龙30mg/kg(稀释至2mL);人组织激肽释放酶组(HTK组),自尾静脉每日注射HTK 17.5×10-3PNAU/kg(稀释至2mL)治疗。在术前及术后第1、3、5、7、9、11、13天各时间点测定坐骨神经功能指数(SFI),术后第14天取出坐骨神经干,测定动作电位传导速度(NCV)。结果:三组大鼠SFI值在术后第1、3天均为-100左右。自第3天开始,HTK组和SM组SFI恢复的情况要优于对照组,有统计学意义(P<0.05)。在术后第14天HTK组和激素组的NCV值高于对照组,差异有统计学意义(P<0.05)。结论:大鼠经尾静脉注射HTK,可促进坐骨神经损伤修复,神经功能恢复快。更多还原

【Abstract】 AIM : To study the repair effect of human tissue kallikrein(HTK) on sciatic nerv-ous of rats.METHODS : Thirty-six adult male SD rats weighted 180-220g were randomly di-vided into 3groups.The control group was just injected with 2mL salt water by caudal vain every day.The SM group was injected with methylprednisolone 30 mg / kg(2 mL) by caudal vain every day.The HTK group was injected with human tissue kallikrein 17.5×10-3 PNAU / kg(2 mL) by caudal vain every day.The sciatic functional index(SFI) at the day 1,3,5,7,9,11 and 13after operation in all rats were calculated.The nerve conduction velocity of the sciatic nerve at the 14 th day postoperation was observed.RESULTS : All rats showed-100at day 1 and 3.The HTK group and the SM group showed a statistically significant improvement over the control group from the 3 th day postoperation.The NCV of the HTK group and the SM group were greater than the control group(P< 0.05).CONCLUSION : The repair effect of human tissue kallikrein(HTK) on sciatic nervous of rats was effective.更多还原