【摘要】 目的:观察木瓜蛋白酶体外对凝血功能的抑制作用,并探讨其可能机制。方法:将不同剂量木瓜蛋白酶分别与贫血小板血浆(PPP)和富血小板血浆(PRP)作用,分为生理盐水组、10U/L组和20U/L组,分别以血凝仪测定PPP和PRP的凝血酶原时间(PT)和活化部分凝血活酶时间(APTT),以血气分析仪和血凝仪分别测定PPP的Ca2+浓度和凝血因子V、VII、VIII、IX、X和XI活性(FV:C、FVII:C、FVIII:C、FIX:C、FX:C和FXI:C);将新鲜全血与前述3种浓度木瓜蛋白酶作用,采用硅化管法测定全血凝血时间(CT)。同时测定0、20、40、60和80U/L木瓜蛋白酶PPP的PT和APTT值。结果:10 U/L和20U/L木瓜蛋白酶组PPP和PRP的PT和APTT值、全血CT值分别显著高于生理盐水组和10U/L木瓜酶组(P<0.01),FV:C和FVIII:C水平分别显著低于生理盐水组和10U/L木瓜酶组(P<0.05);三组PPP与PRP之间PT和APTT值、各组间Ca2+浓度以及其余凝血因子活性差异均无显统计学意义(P>0.05)。PPP的PT和APTT值均与木瓜蛋白酶剂量呈显著正相关(r=0.995和0.991,P<0.01)。结论:木瓜蛋白酶可通过抑制凝血因子V和VIII活性,从而对凝血功能有剂量依赖性的抑制作用,具有抗凝的功效。更多还原

【Abstract】 AIM : To observe the in vitro effect of papain on blood coagulation function,and to explore the possible mechanism.METHODS : Human platelet poor plasma(PPP) and platelet rich plasma(PRP) were mixed with different dose of papain which were assigned into three groups(group 0U / L,group 10U / L and group 20 U / L).PPP and PRP were measured for prothrombin time(PT) and actvated partial thromboplastin time(APTT) with blood coagulation analyzer,the PPP was also measured for Ca 2+ and activity of coagulable factor V,VII,VIII,IX,X and XI(FV : C 、 FVII : C 、 FVIII : C 、 FIX : C 、 FX : C and FXI : C) with blood-gas analyzer and blood coagulation analyzer,respectively.Fresh blood from volunteers was mixed with the three dose of papain,the clotting time(CT) was detected with silicified tube method.The PPP with 0,20,40,60and 80 U / L of papain also was measured for PT and APTT.RESULTS : At group 10U / L papain and group 20U / L papain,PT and APTT of PPP and PRP as well as CT of whole blood was significantly higher,but FV : C and FVIII : C activity was remarkably lower than that at group physiological saline solution and group 10 U / L papain,respectively.There was no statistical difference for PT and APTT between PPP and PRP at the three groups,for Ca 2+ concentration and other four coagulable factors activity between the three groups one another.Either PT or APTT level at group 0,20,40,60and 80U / L of papain showed positive correlation with papain dose.CONCLUSION : Papain has dose-dependent inhibitory effect on blood coagulation mainly through inhibition of coagulable facor V and VIII activity.Papain has the efficacy of anticoagulation更多还原