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标本保存对实时荧光定量HCV-RNA检测的影响
Impact of the specimen preservation on the detection of serum HCV RNA using FQ-RT-PCR
【摘要】 目的研究标本保存对实时荧光定量HCV-RNA检测结果的影响。方法收集HCV阳性血清标本42份并平均分成6份分别置于室温、4℃和-20℃保存1d和1周后用荧光定量PCR法检测结果,行统计学分析。结果血清在室温、4℃和-20℃条件下保存1d和1周对HCV RNA的荧光定量检测结果无统计学差异。结论保存温度和保存时间对HCV RNA的定量检测结果无影响。
【Abstract】 Objective To investigate the impact of various storage conditions of serum samples on HCV RNA detection. Methods Real-time fluorenscent quantitative PCR detected 42 examples have been diagnosed in patients with HCV. Result The results of HCV RNA in serum samples,which stored 1 day and 7 days at room temperature, 4 ℃ and-20℃, showed no significant differences. Conclusion Save temperature and save time have no effect on test results.
【关键词】 标本保存；
HCV-RNA；
FQ-RT-PCR；
【Key words】 The preservation of the specimen； Hepatitis C virus RNA； Fluorescence quantitative PCR；
【Key words】 The preservation of the specimen； Hepatitis C virus RNA； Fluorescence quantitative PCR；
- 【文献出处】 中国医疗前沿 ,National Medical Frontiers of China , 编辑部邮箱 ,2013年22期
- 【分类号】R440
- 【被引频次】3
- 【下载频次】127