【摘要】 目的了解产超广谱β-内酰胺酶(ESBLs)大肠埃希菌和克雷伯菌的主要基因型分布特点。方法用表型确证试验筛选出临床标本中产ESBLs的大肠埃希菌和克雷伯菌114株。应用PCR方法扩增产酶株的TEM、SHV和CTX-M基因。结果PCR结果显示TEM、SHV和CTX-M基因的总阳性率分别为40.4%、20.7%和83.3%,其中大肠埃希菌分别为48.1%、1.3%和93.5%,肺炎克雷伯菌分别为24.3%、81.1%和62.2%。46.8%的大肠埃希菌和51.4%的肺炎克雷伯菌同时携带多个基因。结论产ESBLs大肠埃希菌的主要基因型为CTX-M,肺炎克雷伯菌主要基因型为SHV。大多数菌株同时携带多个基因。更多还原

【Abstract】 Objective To investigate the genotypic character of clinical isolates producing extended spectrum betalactamases(ESBLs).Methods ESBLs producing Escherichia coli and Klebsiella pneumoniae isolates were indentified by phenotyic confirmatory test.PCR were using to detect TEM,SHV and CTX-M genes.Results The positive rates of TEM,SHV and CTX-M genes of these isolates were 40.4%,20.7% and 83.3% respectively.Those of Escherichia coli were 48.1%,1.3% and 93.5% respectively.Those of Klebsiella pneumoniae were 24.3%,81.1% and 62.2% respectively.46.8% of Escherichia coli and 51.4% of Klebsiella pneumoniae had more than one genotype.Conclusions The major genotype of ESBLs-producing Escherichia coli was CTX-M and which of Klebsiella pneumoniae was SHV.Many isolates had more than one genotype.更多还原