【摘要】 目的采用雌性SD大鼠建立单程长时应激(SPS)模型,从表观效度和预测效度2方面考察其是否能作为创伤性应激障碍的动物模型。方法雌性大鼠依次连续给予固定束缚(2 h)、强迫游泳(20 min)和乙醚麻醉各1次作为应激因子制备SPS模型,随后连续2周内每天1次ig给予舍曲林10 mg.kg-1。2周后给予足底电击刺激,次日环境重现,测定呆滞行为时间;高架十字迷宫实验测定进入开臂次数百分比和开臂滞留时间百分比;开场实验观察爬格次数和站立次数。结果与正常对照组比较,模型组大鼠呆滞行为时间明显延长(P<0.01),进入开臂次数百分比和开臂滞留时间百分比显著降低(P<0.01);与模型组相比,舍曲林组大鼠呆滞行为时间显著降低,进入开臂次数百分比和开臂滞留时间百分比显著增加(P<0.05,P<0.01);各组爬格次数和站立数与正常对照组无显著性差异。结论通过SPS成功诱导建立雌性大鼠创伤性应激障碍动物模型。更多还原

【Abstract】 OBJECTIVE To establish a single prolonged stress(SPS) model in female rats and to study whether it can be successfully developed as a post-traumatic stress disorder(PTSD) animal model according to the theory of face validity and predictive validity.METHODS The female rats were subjected to three stresses(restraint for 2 h,forced swim for 20 min,and ether anesthesia) successively,which were used as the stress factors of the SPS model.Sertraline 10 mg·kg-1 was ig given to rats,once daily,for 14 d.After that,the female rats were exposed to foot shock.Twenty-four hours after foot shock conditioning,their freezing behavior was measured.The percentage of times of open arm entries and the percentage of time spent in the open arms was assessed in the elevated plus maze test.The number of crossing and rearing was also measured in the locomotor activity.RESULTS Female rats subjected to SPS showed a significant increase in contextual freezing compared to rats without SPS(P<0.01).Rats exposed to SPS significantly decreased the percentage of time spent in open arms in the elevated plus maze test and the times of open arm entries(P<0.05,P<0.01).The chronic sertraline treatment could reverse these PTSD behaviors in female SPS rats.There was no significant difference in the rat locomotor activity.CONCLUSION A single prolonged stress model can be successfully established in female rats.更多还原