【摘要】 目的探讨实验性偏头痛大鼠痛觉超敏行为及颈髓C1、C2后角环氧化酶2(COX-2)在偏头痛大鼠中枢敏化过程中的表达。方法健康成年SD大鼠30只,随机均分五组:空白对照(A)组、假手术(B)组、生理盐水(C)组、新型致炎剂(NIS)4d(D)组和NIS 7d(E)组。用Von-Frey纤维丝测定各组动物眶周痛觉阈值,免疫组化、Western blot检测大鼠颈髓C1、C2后角COX-2表达的变化。结果 C组大鼠的疼痛行为与A组相仿(P>0.05)。E组大鼠建模后第4天眶周痛觉阈值明显低于A组(P<0.05)。D组、E组COX-2阳性细胞计数均高于C组(P<0.01)。E组大鼠颈髓后角COX-2蛋白表达量明显高于A组(P<0.05)。结论 NIS硬脑膜给药能有效诱导偏头痛大鼠产生痛觉超敏,并与大鼠颈髓后角COX-2表达的增加密切相关。更多还原

【Abstract】 Objective To observe the effects of new inflammatory soup(NIS)on the hypersensitivity behaviors in experimental rats with migraine and the expression of cyclooxygenase-2(COX-2)in the posterior horn of cervical spinal cord.Methods Thirty male SD rats were equally randomized into 5groups of A(blank control),B(sham surgery B),C(saline-treated),D(on the 4th d after NIS-infused)and E(on the 7th d after NIS-infused).Von-Frey hair was used to monitor the pain threshold of allodynia around orbital skin.The expression of COX-2in cervical spinal cord posterior horn was detected by immunohistochemistry and Western blot.Results There was no significant difference in the hypersensitivity behaviors between groups of A and C(P>0.05).The pain threshold of allodynia on the 4th d after NIS-infused was lower in group E than that in group A(P<0.05).The number of COX-2positive cells was more in groups of D and E than that in group C(P<0.01).The expression of COX-2protein in the cervical spinal cord posterior horn was higher in group E than that in group A(P<0.05).Conclusion Dural matter infusion of NIS can induce mechanical allodynia in rats,which is associated with an increased expression of COX-2in the posterior horn of cervical spinal cord.更多还原