【摘要】 目的观察ω-3多不饱和脂肪酸(ω-3 PUFA)对严重烫伤大鼠肝脏及炎症反应的影响,并就其对肝脏保护作用的机制进行探讨。方法 72只SD大鼠随机分为3组:假烫组8只、对照组(中长链脂肪酸组)32只和治疗组(ω-3 PUFA组)32只。采用大鼠30%总体表面积(TBSA)Ⅲ度烫伤模型。对照组每天按时经尾静脉注射与治疗组相同热量的中长链脂肪酸;治疗组经尾静脉注射ω-3 PUFA 2 ml/(kg.d);假烫组不予任何处置。观察假烫组、对照组和治疗组烫伤后1、4、7、10 d血清内毒素(ET)、前列腺素E2(PGE2)的含量;免疫组化检测肝组织核因子-κB(NF-κB)p65的活性水平;RT-PCR检测肝组织肿瘤坏死因子α(TNF-α)mRNA的表达,各个时间点均处死8只大鼠。结果①在伤后所有时间点,对照组、治疗组血清ET、PGE2水平明显高于假烫组(P<0.01);治疗组血清ET、PGE2水平比对照组降低,伤后4、7、10d与对照组比较,差异有统计学意义(P<0.05)。②伤后对照组、治疗组肝组织NF-κBp65表达比假烫组增强;在伤后4、7 d,与对照组比较,治疗组肝组织NF-κBp65表达减弱(P<0.05)。③对照组、治疗组在烫伤后各时间点,肝组织TNF-αmRNA表达较假烫组明显升高(P<0.01),与对照组比较,在烫伤后4、7 d,治疗组肝组织TNF-αmRNA的表达明显降低(P<0.05)。结论①ω-3 PUFA能降低烫伤后血清ET、PGE2的含量,减轻肝脏组织NF-κBp65和TNF-αmRNA的表达。②ω-3 PUFA减轻严重烫伤后肝组织的损伤。更多还原

【Abstract】 Objective To study the influence of ω-3 polyunsaturated fatty acids(ω-3 PUFA) on liver and inflammatory reaction in serious scalded rats and explore its mechanism.Methods A total of 72 SD rats with 30% total body surface area(TBSA) third-degree scalded were optional separated into 3 groups:the phony scald group 8,the control group(middle-long-chain fatty acids group) 32 and treatment group(ω-3 PUFA group)32.Rats in the control group were injected middle-long-chain fatty acids with equivalent calories by tail vein,and rats in the treatment group were injected ω-3 PUFA 2 ml/(kg·d) by tail vein on time for everyday.The phony scald group,the control group and treatment group 1,4,7,10 d were measured respectively the levels of serum endotoxin(ET),prostaglandinE2(PGE2)and expression of nuclear factor-kappa B(NF-κB)p65 in liver tissue was detected by immunohistochemistry staining.Expression of liver tissue TNF-α mRNA was measured by RT-PCR technology.Each subgroup had 8 rats.Results ① Serum ET,PGE2 were obvious higher than the phony scald group at each time point in the control group and treatment group(P<0.01).The levels in the treatment group were lower than in the control group,and were significantly different in 4,7,10 d after scalded(P<0.01).② Compared with the phony scald group,the expression of liver tissue NF-κBp65 was increased in the control group and treatment group;Compared with the control group,the activity of NF-kBp65 was significantly decreased after scalded 4,7 d in the treatment group(P<0.05).③ The control group and treatment group at all time points after scalded the expression of liver tissue TNF-α mRNA was significantly higher than the phony scald group(P<0.01);Compared with the control group,after scalded 4,7d,the expression of liver tissue TNF-α mRNA was significantly reduced in the treatment group(P<0.05).Conclusion ① ω-3 PUFA can reduce the levels of serum ET,PGE2,relieve the expression of liver tissue NF-κBp65 and TNF-α mRNA.② ω-3 PUFA can alleviate the damage of liver tissue after scald.更多还原