【摘要】 目的研究围生期高剂量双酚A(BPA)暴露对仔鼠生命早期调节性T细胞(Treg)的影响,初步探讨BPA发育免疫毒性的机制。方法将确认妊娠的ICR母鼠随机分为空白对照组、溶剂对照组、BPA低剂量组(0.125 g/L)、BPA中剂量组(0.5 g/L)和BPA高剂量组(2 g/L);母鼠自妊娠第0天(GD 0)至仔鼠出生后第7天(PND 7)经饮水染毒。PND 7时处死仔鼠,无菌取胸腺,流式细胞仪(FCM)检测胸腺Treg细胞数量,RT-PCR检测Treg细胞特征性转录因子Foxp3 mRNA表达水平。结果与空白对照组及溶剂对照组比较,BPA高、中剂量组仔鼠体重在PND 1、PND 4和PND 7时明显降低(P<0.05);与空白对照组及溶剂对照组比较,BPA高、中、低3个剂量组仔鼠胸腺Treg细胞数量明显减少(P<0.01);与空白对照组及溶剂对照组相比较,3个BPA暴露组仔鼠Foxp3 mRNA表达水平明显减少,差异均有统计学意义(P<0.01)。结论围生期高剂量BPA暴露可明显影响仔鼠生命早期体内Treg细胞,从而影响机体免疫功能。更多还原

【Abstract】 Objective To study the effect of high-dose bisphenol A(BPA)exposure during perinatal on regulatory T cells(Treg)of offsprings in early life,and explore the mechanism of developmental immunotoxicity of BPA.Methods The pregnant female ICR mice were intaked BPA(0.125,0.5,2 g/L)by drinking water till the 7th day after the offsprings were born(PND 7),water and acetone(4%)were served as blank and vehicle control.And offsprings were sacrificed then thymus were taken by asepsis,the proportion of Treg cells and Foxp3 mRNA expression level in thymus were measured by flow cytometry and real-time PCR.Results In the middle/high-dose groups weight of offspring at three time points(PND 1,PND 4,PND 7)decreased significantly compared with the control groups(P<0.05);compared with the control group and the vehicle group,Treg cells of BPA treated groups were significantly decreaed(P<0.01),and thymic Foxp3 mRNA expression level of BPA treated groups were significantly reduced compared with the control group(P<0.01).Conclusion High dose of BPA exposure during perinatal period can affect the proportion of Treg cells significantly in early life of the offsprings,thus affecting the body immune function.更多还原