【摘要】 对芜菁见光肉质根表皮3 798条无冗余EST的SSR信息进行了分析,共检出111个由13种重复基元所构成的SSR,检出率为2.92%,平均相距11.974kb出现1个SSR。其中,二核苷酸和三核苷酸重复基元的EST-SSR占主导地位,分别占被检SSR总数的54.05%和45.05%;AG/CT和AAG/CTT基元的重复分别是二核苷酸和三核苷酸类型重复中的优势重复,分别占二核苷酸和三核苷酸重复类型的88.33%和32.00%;这些EST-SSR中属于具有中等程度多态性的Type II类型占93.69%,仅少数(<7%)属于具有较高多态性的Type I类型。经过Blast2Go分析,与这些EST-SSR相关联的EST参与10个不同代谢途径。更多还原

【Abstract】 The SSR information in 3 798 irredundant ESTs from the hypocotyl of turnip(Brassica rapa subsp.Rapa)exposed to light were investigated.As the results,111SSRs accounting for 2.92% of ESTs were mined out,which with 13 kinds of repeat motifs.The average distance between SSRs was 11.974kb. In the hypocotyl exposed to light,EST-SSRs with dinucleotide and trinucleotide repeats were major SSRs, accounting for 54.05% and 45.05% in total SSRs,respectively.Among 13 repeat motifs,AG/CT and AAG/CTT motifs were the most frequent motifs,accounting for 88.33% and 32.00% in dinucleotide and trinucleotide repeats,respectively.Among the EST-SSRs,about 93.69% of SSRs were belong to the medium polymorphic Type II,and others were belong to high polymorphic Type I.In addition,the results by Blast 2 Go analysis revealed that the ESTs involving SSRs from turnip swollen hypocotyl exposed to light were related with 10 different metabolic pathways.更多还原