【摘要】 通过对乳酸菌复合系SFC-2培养基的组分进行响应面优化,从而得出SFC-2优化培养基配方为白砂糖10g/L、蛋白胨31.36g/L、牛肉膏15.57g/L、酵母膏10g/L、乙酸钠7.3g/L、柠檬酸三铵4g/L、K2HPO44g/L、MgSO4.7H2O 0.58g/L、MnSO4.H2O 0.25g/L。SFC-2在此培养基中经30℃,16h培养,其活菌数可达到4.0×108 cfu/mL,较MRS-S中(8.2×107 cfu/mL)提高近5倍。更多还原

【Abstract】 The medium components for LAB community SFC-2 were optimized by response surface methodology,which contained white granulated sugar 10 g/L,peptone 31.36 g/L,beef extract 15.57 g/L,yeast extract 10 g/L,sodium acetate 7.3 g/L,triammonium citrate 4 g/L,K2HPO4 4 g/L,MgSO4·7H2O 0.58 g/L,MnSO4·H2O 0.25 g/L.After cultivation in optimization medium for l6 h at 30 ℃,the viable count of SFC-2 was 4.0×108 cfu/mL,which was about 5 times higher than that in MRS-S(8.2×107 cfu/mL).更多还原