【摘要】 目的 研究雌激素受体GPR30激动剂G-1对大鼠心肌缺血/再灌注损伤的保护作用。方法 32只雌性大鼠随机分为假手术组（Sham组）、卵巢切除组（OVX组）、缺血/再灌注组（I/R组）和G-1组,每组8只。除Sham组外,其他各组大鼠均切除双侧卵巢,G-1组卵巢切除后予G-1皮下注射2周,剂量为120μg·kg^-1·d^-1。2周后取各组实验动物心脏,Langendorff灌流,记录左心室收缩压（LVSP）、左心室舒张末压（LVEDP）、左心室压变化最大速率(±dp/dt_max);I/R组和G-1组进行离体心脏缺血/再灌注;检测各组心肌梗死面积,TUNEL检测心肌细胞凋亡。结果 与Sham组和OVX组相比,I/R组左心室发展压（LVDP）和±dp/dt_max降低,LVEDP升高,心肌梗死面积增加,凋亡心肌细胞数量增多;G-1组心功能改善,心肌梗死面积减小,心肌细胞凋亡数量减少。结论 G-1通过抑制细胞凋亡减轻大鼠心肌缺血/再灌注损伤。更多还原

【Abstract】 Objective To investigate the protective effects of the estrogen membrane receptor GPR30 agonist G-1 on myocardial ischemia/reperfusion injury in rats.Methods A total of 32 female SD rats were randomly divided into four groups（n=8 each）:a sham-operated control（Sham） group,an ovariectomized（OVX） group,an ischemia/reperfusion（I/R） group and a G-1 group.Bilateral ovariectomy was performed in all groups except the Sham group.A selective agonist of GPR30,G-1(120 μg·kg^-1·d^-1) was subcutaneously injected into the rats of the G-1 group for 2 weeks.Two weeks later,the Langendorff assay was performed in each group to detect left ventricular systolic pressure（LVSP）,left ventricular end-diastolic pressure（LVEDP） and the maximal rate of left ventricular pressure rise(± dp/dt_max).Ischemia/reperfusion in isolated heart was performed in the I/R and G-1 groups.Myocardial infarction size was measured.TUNEL was used to examine cardiomyocyte apoptosis.Results Compared with the Sham and OVX groups,the I/R group showed decreased levels of left ventricular developed pressure（LVDP） and ±dp/dt_max but an increased level of LVEDP,with an enlarged infarct size and an increased number of apoptotic cells.The rats in the G-1 group showed improvement in cardiac function and reduction of myocardial infarct size and the number of apoptotic cardiomyocytes.Conclusion G-1 can alleviate myocardial ischemia/reperfusion injury in rats through inhibition of cardiomyocyte apoptosis.更多还原