【摘要】 目的 比较银杏叶提取物及其主要成分银杏黄酮和银杏内酯B对小鼠脑缺血/再灌注损伤的保护作用。方法 建立小鼠脑缺血/再灌注损伤模型,观察比较银杏叶提取物、银杏黄酮、银杏内酯B对小鼠存活时间、脑含水量及脑皮质组织中Na⁺-K⁺-ATP酶活性、丙二醛（MDA）含量、超氧化物歧化酶（SOD）活性的影响。结果 银杏叶提取物300 mg·kg^-1能显著延长小鼠的存活时间,降低小鼠脑含水量,提高脑皮质组织中Na⁺-K⁺-ATP酶活性,抑制MDA含量增加和SOD活性降低（P<0.01）。银杏内酯B和银杏黄酮亦有相似的作用,其中银杏内酯B对皮质Na⁺-K⁺-ATP酶活性的影响显著（P<0.01）,而银杏黄酮能显著降低皮质中MDA的含量（P<0.01）。结论 银杏叶提取物、银杏内酯和银杏黄酮对小鼠全脑缺血/再灌注损伤均具有保护作用,银杏内酯B较银杏黄酮有较好的抑制脑水肿、提高Na⁺-K⁺-ATP酶活性的作用,而银杏黄酮抗氧化作用较优于银杏内酯B。更多还原

【Abstract】 Objective To compare the protective effects of Ginkgo Biloba extract（GbE）,Ginkgo flavonoids（GF）and ginkgolide B（GB） on global ischemia/reperfusion injury in mice.Methods The mice global ischemia/reperfusion injury model was established.Mice were pretreated with GbE,GF and GB separately.The survival time,brain water content,activity of Na⁺-K⁺-ATPase,MDA content,activity of SOD in mice cerebral cortex were detected after 20 min ischemia and 24 h reperfusion.Results 300 mg·kg^-1 of GbE could obviously extend the mice survival time,reduce the brain water content,enhance Na⁺-K⁺-ATPase activity,suppress the MDA content and increase the SOD activity in mice cerebral cortex（P <0.01）.GB and GF had the similar tendency,in which GB significant enhanced Na⁺-K⁺-ATPase activity in the cortex and decreased brain water content（P <0.01）.GF reduced the content of MDA in the cortex significantly（P <0.01）.Conclusion GbE,GF and GB can protect the mice against global cerebral ischemia/reperfusion injury.Compared with GF,GB has a better effect against cerebral edema and enhance the Na⁺-K⁺-ATPase activity.However,GF has the better antioxidant effect than GB.更多还原