【摘要】 目的 观察FOXO3a基因沉默后,三氧二砷（As₂O₃）对人胃癌MGC803细胞FOXO3a和血管内皮生长因子（VEGF）表达的影响。方法 采用脂质体转染小干扰RNA（siRNA）方法降低胃癌MGC803细胞FOXO3a的表达。采用反转录聚合酶链反应（RT-PCR）法检测FOXO3a和VEGF mRNA的表达;采用Western blot法检测FOXO3a和VEGF蛋白的表达。结果 与对照组相比,As₂O₃促进胃癌MGC803细胞内FOXO3a蛋白和mRNA的表达,并抑制VEGF蛋白和mRNA的表达,FOX03a siRNA处理后明显抑制FOXO3a蛋白与mRNA表达且增加VEGF蛋白和mRNA表达。结论 As₂O₃可能是通过促进MGC803细胞中FOXO3a的表达引起VEGF表达的降低。更多还原

【Abstract】 Objective To investigate the effect of arsenic trioxide（As₂O₃） on expressions of FOXO3a and vascular endothelial growth factor（VEGF） in gastric cancer MGC803 cells after silencing of FOXO3a gene.Methods FOXO3a specific small interfering RNA（siRNA） was transfected into the human gastric cancer MGC803 cells by liposome method.The FOXO3a and VEGF mRNA in MGC803 cells were detected by reverse transcription-polymerase chain reaction（RT-PCR）.Western blot was employed to detect the expressions of FOXO3a and VEGF proteins in MGC803 cells.Results Compared with the control group,As₂O₃ promoted the expression of FOXO3a and inhibited the VEGF expression in mRNA and protein.However,the expression of FOXO3a was lower and VEGF was higher after RNA interference.Conclusion As₂O₃ can inhibit the expression of VEGF via increasing the expression of FOXO3a in human gastric cancer MGC803 cells.更多还原