【摘要】 目的 探讨缺氧诱导因子-1α(HIF-1α)和趋化因子受体4(CXCR4)蛋白在胃癌组织中的表达及与胃癌临床病理因素的相关性。方法 采用免疫组化SP法检测44例胃癌组织、44例相应癌旁组织(距癌组织边缘>5 cm)中HIF-1α和CXCR4蛋白的表达,分析胃癌组织中HIF-1α和CXCR4蛋白表达与胃癌临床病理因素的相关性,并分析HIF-1α和CXCR4蛋白表达之间是否相关。结果 癌旁组织中HIF-1α和CXCR4蛋白均无表达,胃癌组织中HIF-1α和CXCR4蛋白阳性表达率分别为59.1%和63.6%,与癌旁组织比较差异均有统计学意义(P<0.01);HIF-1α蛋白表达与胃癌TNM分期和浸润深度相关(P<0.01),与是否淋巴结转移相关(P<0.05),与性别、年龄、肿瘤直径和分化程度无关;CXCR4蛋白表达与是否淋巴结转移相关(P<0.01),与TNM分期相关(P<0.05),与性别、年龄、肿瘤直径、浸润深度和分化程度无关。胃癌组织中HIF-1α与CXCR4蛋白表达呈正相关(r=0.332,P=0.028)。结论 HIF-1α和CXCR4蛋白表达可能与胃癌的发生有关,胃癌组织中HIF-1α和CXCR4蛋白的高表达预示胃癌恶性程度高,临床分期较晚,可能存在淋巴结转移。联合检测HIF-1α和CXCR4蛋白的表达,有利于评估胃癌的生物学行为,提高预后判断的准确性,并对临床治疗有重要指导意义。更多还原

【Abstract】 Objective To investigate the expression of hypoxia-inducible factor-1α(HIF-1α)and chemokine receptor 4(CXCR4)protein in tissues of gastric cancer and their correlations with clinicopathological features of gastric cancer.Method The expression of HIF-1α and CXCR4 protein in 44 tissues of gastric cancer and 44 adjacent gastric tissues(5 cm away from the tumor)were detected by immunohistochemical SP method.The correlations between the expression of HIF-1α and CXCR4 protein in the tissues of gastric cancer and clinicopathological factors were analyzed.The correlation between the expression of HIF-1α and CXCR4 protein was also analyzed.Results There was no positive expression of HIF-1α and CXCR4 protein in adjacent gastric tissues.The positive expression rates of HIF-1α and CXCR4 protein in tissues of gastric cancer were 59.1% and 63.6% respectively,which were significantly different from that in adjacent gastric tissues(P <0.01).The expression of HIF-1α protein was significantly related to TNM stage and invasive depth(P <0.01),and was closely related to lymph node metastasis(P <0.05),but was not related to gender,age,tumor diameter and differentiation.The expression of CXCR4 protein was significantly related to lymph node metastasis(P <0.01),and was closely related to TNM stage(P <0.05),but was not related to gender,age,tumor diameter,invasive depth and differentiation.There was a positive correlation between the expression of HIF-1α and CXCR4 protein in the tissues of gastric cancer(r=0.332,P=0.028).Conclusion The significantly higher expression of HIF-1α and CXCR4 protein may be related to the carcinogenesis of gastric cancer.The high expression of HIF-1α and CXCR4 protein in tissues of gastric cancer indicates high malignant degree,advanced clinical stage and possible lymph node metastasis.Combined analysis of the expression of HIF-1α and CXCR4 protein has benefit in evaluation of the biological behaviour of gastric cancer,increases the accuracy of prognosis prediction and plays an important guiding role in the clinical treatment.更多还原