【摘要】 目的 对Ad-Wnt3a-GFP穿梭重组体与骨架载体进行原核重组和真核包装。方法 将Ad-Wnt3a-GFP穿梭重组体与骨架载体以电穿孔法共转染至原核包装细胞BJ5183;再将原核重组体用脂质体转入真核包装细胞293H进行包装。结果(1)电泳鉴定得到原核重组体;(2)得到完整的具感染能力的病毒颗粒;(3)病毒颗粒能表达标签蛋白。结论 获得了具有感染能力的病毒颗粒。更多还原

【Abstract】 Objective To construct recombinant Ad-Wnt3a-GFP.Methods The resultant plamid was cotransformed into E.coli.BJ5183 cells with an adenoviral backbone plasmid pAdEasy-1 and then the recombinant plasmid was transfected into adenovirus packaging cell lines HEK 293 cells with Lipofectamine.Results(1) Recombination confirmed by restriction endonuclease analyses.(2) Recombinant adenoviruses were typically generated.(3) Recombinant adenoviruses could express protein GFP.Conclusion Adenovirus partiles capable of infections were obtained.更多还原