【摘要】 目的建立高效液相色谱(HPLC)法测定小鼠血清中痂囊腔菌素A的浓度,考察其在小鼠体内的药动学特征。方法 60只小鼠,雌雄各半,痂囊腔菌素A 30.0 mg灌胃后,于不同时间点分取血浆,采用HPLC分别测定血清样品中痂囊腔菌素A浓度,用DAS 3.0.8软件计算药动学参数。结果痂囊腔菌素A在10.0~800.0μg.L-1内线性关系良好(r=0.999 6),日内和日间RSD<7.0%,提取回收率为89.2%~92.4%,方法回收率为100.1%~100.6%。痂囊腔菌素A在小鼠体内的主要药动学参数:AUC0-t为(16 697.8±873.0)μg.L.h-1,MRT0-t为(3.8±0.0)h,t■z为(3.6±0.0)h,tmax为(1.0±0.0)h,CLz/F为(1.8±0.0)L.h-1,ρmax为(5 889.3±135.9)μg.L-1。结论本法简便、灵敏、重现性好。痂囊腔菌素A在小鼠体内药动学过程符合二室模型。更多还原

【Abstract】 AIM To establish a HPLC method for determining the serum concentration of elsinochrome A and to investigate its pharmacokinetic in mice.METHODS After the plasma from 60 mice(half male and half female) with intragastric were collected in different time point,elsinochrome A in serums were collected and determined by HPLC.The pharmacokinetic parameters were calculated by DAS 3.0.8.RESULTS The good linear range of elsinochrome A were from 10.0-800.0 μg.L-1(r = 0.999 6).The limit of quantitation(LOQ) was 1.0 μg.L-1.The RSD(%) of intra-and inter-day were all below < 7.0%.The extraction recoveries were in the range of 89.2%-92.4% and the method recoveries were 100.1%-100.6%.The main pharmacokinetic parameters of mice were as follows: AUC 0-t =(16 697.8 ± 873.0) μg.L.h-1,MRT 0-t =(3.8 ± 0.0) h,t ■z =(3.6 ± 0.0) h,t max =(1.0 ± 0.0) h,CLz/F =(1.8 ± 0.0) L.h-1,ρ max =(5 889.3 ± 135.9) μg.L-1.CONCLUSION This method is convenient,sensitive and reproducible.The pharmacokinetic character of Elsinochrome A in mouse after oral showes linear and conforms to the two compartment models.更多还原