【摘要】 本研究旨在探讨人类非典型HLA抗原E(HLA-E)对同胞全相合造血干细胞移植(HSCT)后巨细胞病毒(CMV)感染的影响。以2005年1月至2011年1月在我院行HLA同胞全相合HSCT患者119例为研究对象,采用PCR和测序方法检测供、受体HLA-E基因多态性。结果表明,HLA-E*0101/0101纯合型占20.17%,HLA-E*0103/0103纯合型占27.73%,HLA-E*0101/0103杂合型占52.10%;HLA-E*0101/0101纯合子组CMV感染15例,CMV感染率为62.50%;HLA-E*0103/0103纯合子组CMV感染16例,CMV感染率为48.48%;HLA-E*0101/0103杂合子组CMV感染20例,CMV感染率为32.25%。含HLA-E*0103基因组与HLA-E*0101/0101纯合子组患者CMV感染率比较,差异有统计学意义(P=0.0295),与CMV疾病的发生率比较,差异仍有统计学意义(P=0.0074)。结论:HLA-E基因多态性对同胞全相合移植后CMV感染和疾病的发生存在影响。更多还原

【Abstract】 This study was aimed to explore the potential association of HLA-E polymorphism with the incidence of cytomegalovirus(CMV) infection after HLA-matched hematopoietic stem cell transplantation,119 HLA-genoidentical sibling pairs for HLA-E polymorphism were analyzed,HLA-E DNA was amplified by polymerase chain reaction(PCR),and the amplified DNA products was also sequenced directly after purification to confirm the genotype.The results showed that the homozygous HLA-E*0101/0101 accounted for 20.17%,the homozygous HLA-E*0103/0103 accounted for 27.73%;heterozygous HLA-E*0101/0103 accounted for 52.10%;in homozygous HLA-E*0101/0101 group,15 cases were infected with CMV and the CMV infection rate was 62.50%;in homozygous HLA-E*0103/0103 group,16 cases were infected with CMV and the CMV infection rate was 48.48%;in heterozygous HLA-E*0101/0103 group 20 cases were infected with CMV and the CMV infection rate was 32.25%.As compared with the homozygous HLA-E*0101/0101 group,the CMV infection rate in HLA-E*0103 group displays statistical significance(P=0.0295).The CMV infection rate occured higher and its significance is statistical(P=0.0074).It is concluded that the HLA-E gene polymorphism is associated with CMV infection after HLA-genoidentical bone marrow transplantation.更多还原