【摘要】 目的探讨快速有效的阴道毛滴虫染色方法,以满足实验教学需求。方法将pH 6.4～6.8肝浸汤培养基转种2～3次的无菌阴道毛滴虫,在春秋季25℃和冬季10℃室温条件下,用体积分数为2%、5%、8%的Giemsa染液分别染色15、20、25、30、35、40 min,观察染色情况。结果体积分数5%的Giemsa染液在春秋季室温25℃染色20 min、冬季室温10℃染色25 min时,阴道毛滴虫外形清晰呈梨形,细胞核呈紫红色,细胞质呈浅蓝色,波动膜、鞭毛和轴柱均可以观察到。结论采用体积分数5%、pH 6.4～6.8的Giemsa染液,在室温25℃时染色20 min或室温10℃染色25 min染出的阴道毛滴虫标本比较理想,符合教学需求。更多还原

【Abstract】 Objective To explore more fast and effective Trichomonas vaginalis dyeing method for experimental teaching.Methods Taking the Trichomonas vaginalis which had already inoculation for 2-3 times in liver soup medium with pH value for 6.4-6.8,dying under 25 ℃ room temperature in the spring /autumn and 10 ℃ room temperature in the winter respectively,by a concentration of 2%,5% and 8% Giemsa dyeing solution,and the dyeing time were 15,20,25,30,35 and 40 minutes respectively.The staining state was observed.Results The appearance of Trichomonas vaginalis was cleared pear-shaped,purple cell nucleus and pale blue cytoplasm when dyeing 20 minutes in spring/autumn under 25 ℃ room temperature and 25 minutes in winter under 10 ℃ room temperature by volume ratio 5% Giemsa dyeing solution.The undulating membrane,mastigont and axon cylinder can be observed.Conclusion The dyeing result of Trichomonas vaginalis specimen is ideal with the pH value 6.4-6.8,volume ratio 5% Giemsa dyeing solution dyeing 20 minutes in spring/autumn under 25 ℃,and dyeing 25 minutes in winter under 10 ℃.The specimen meet the teaching demand.更多还原