【摘要】 目的 研究缺血后处理对于肺缺血-再灌注损伤的保护作用。方法 60只雄性SD大鼠随机分为2个模型制作组,每个模型制作组30只随机分为5组:假手术组（S）、缺血-再灌注（1/R）45 min组和120 min组,以及缺血后处理（IPO）45 min组和120 min组。用免疫组化法测定肺组织中肺表面活性蛋白A（SP-A）的表达水平,动脉血行动脉血气分析,测定肺组织的通透性(¹²⁵Ⅰ标记牛血清白蛋白的漏出量表示),光镜及电镜下观察肺组织的病理形态变化。结果 IPO组与I/R组相比较,SP-A表达水平明显升高（P<0.01）,血气分析结果明显改善,肺组织的通透性明显降低（P<0.01）,肺组织损伤减轻。结论 缺血后处理可以减轻肺缺血-再灌注损伤,主要表现为肺组织氧合的改善,尤其是减轻组织水肿和减少Ⅱ型肺泡上皮细胞及肺泡表面活性物质的破坏。更多还原

【Abstract】 Objective To investigate the effect of ischemia post-conditioning on the rat lung during the ischemia-reperfusion injury by measuring the expression levels of SP-A of the lung tissue and the variation of arterial blood gas.The permeability and the pathological changes of the lung tissue were observed.Methods Sixty male Sprague-Dawley rats were randomized into two model built groups with 30 animals in each group,then each model built group were randomized into five groups with 6 animals in each group:（l）sham operation group（S）.（2） I/R 45 mins group（lR45）.（3）I/R 120 mins group（IR120）.（4）Post-conditioning 45mins group（P45）.（5）post-con-ditioning 120 mins group（P120）.The expression levels of SP-A of lung tissue were detected by immuno-histochemical stain.Arterial blood gas analysis was conducted.The permeability of lung tissue was determinated(indicating by the leakage of ^l25I bovine serum albumin).The pathological changes of the lung tissue were observed under light microscopy and electron microscopy.Results In IPO group,the level of SP-A in lung tissue increased markedly compared with I/R group.The permeability of lung tissue decreased significantly,and the pathologic lesion of lung tissues were slight.Conclusion Post-conditioning could reduce lung I/R injury,the lung protection of IPO show by improvement of the oxygenation,relieve of lung tissue edema and inhibition of the destruction of alveolar epithelial cell type Ⅱ and alveolar surfactant especially SP-A.更多还原