【摘要】 目的:探讨人工亚冬眠疗法对兔急性心肌梗死(AMI)的心肌保护作用和可能的机制。方法:将24只兔随机均分为假手术组、AMI对照组(对照组)和AMI亚冬眠组,观察其体温、心率和呼吸频率变化,以免疫抑制法测定血清肌酸激酶同工酶(CK-MB)含量,以高效液相色谱-电化学检测法测定血浆去甲肾上腺素(NE)含量,比较两AMI组的心肌梗死面积占左心室面积的百分比(I/LV)和凋亡细胞指数(AI)。结果:AMI模型制备后4h,与对照组相比,亚冬眠组体温明显下降[(37.333±0.427)℃比(34.988±0.488)℃],心率和呼吸频率无明显变化,CK-MB[(759.863±64.111)U/L比(560.225±65.837)U/L]和NE[(200.475±43.581)ng/ml比(143.813±43.383)ng/ml]浓度显著下降,I/LV[(19.281±1.687)%比(16.263±2.275)%]和AI[(36.636±1.671)%比(34.521±1.356)%]明显减少(P<0.05或<0.01)。结论:人工亚冬眠疗法通过降低应激反应、诱导低温和抑制细胞凋亡等方式,对兔急性心肌梗死有明显的心肌保护作用。更多还原

【Abstract】 Objective:To explore myocardial protection effect of artificial subhibernation therapy on rabbits with acute myocardial infarction(AMI) and its possible mechanism.Methods:A total of 24 rabbits were randomly and equally divided into sham operation group,AMI control group and AMI subhibernation group.Changes of their body temperature,heart rate and respiratory frequency were observed.Serum concentration of creatine kinase isoenzyme(CK-MB) was measured by immunosuppression method,and plasma concentration of norepinephrine(NE) was measured by high performance liquid chromatography-electrochemical method.Percentage of myocardial infarct area in left ventricular area(I/LV) and apoptosis index(AI) were compared between two AMI groups.Results:Compared with control group4 h after establishment of AMI model,there were significant decrease in body temperature [(37.333±0.427) ℃ vs.(34.988±0.488) ℃ ],I/LV [(19.281±1.687) % vs.(16.263±2.275) % ] and AI [(36.636±1.671) % vs.(34.521±1.356) % ],and concentration of CK-MB [(759.863±64.111) U/L vs.(560.225±65.837) U/L ] and NE [(200.475±43.581) ng/ml vs.(143.813±43.383) ng/ml ] in subhibernation group,P<0.05,or<0.01,but there were no significant changes in heart rate and respiratory frequency between two groups.Conclusion:Artificial subhibernation therapy possesses significant myocardial protection effect on rabbits with acute myocardial infarction through reducing stress response,inducing low temperature and inhibiting cell apoptosis etc.更多还原