【摘要】 目的观察并比较日本血吸虫可溶性成虫抗原(SWA)及虫卵抗原(SEA)在诱导CD4+T细胞分化中的不同作用。方法分别用SWA、SEA免疫C57BL/6小鼠后分离脾细胞,或用SWA、SEA体外刺激正常小鼠来源的脾细胞后,采用流式细胞术(FCM)检测脾细胞中产生IFNγ及IL 4的细胞比例,以及CD4+T细胞中Th1、Th2细胞亚群的比例。结果SWA比SEA更能优势诱导CD4+T细胞产生IFNγ(P<0.05),SEA比SWA更能优势诱导CD4+T淋巴细胞产生IL 4(P<0.05)。结论SWA较SEA更能优势诱导Th1细胞分化,SEA较SWA更能优势诱导Th2细胞分化。更多还原

【Abstract】 Objective To investigate and compare the different effects of soluble adult worm antigen(SWA) and soluble egg antigen(SEA) of Schistosoma japonicum on the differentiation of the splenocytes and CD4 + T cells of mice.Methods The spleno cytes and CD4 + T cells were prepared from the spleens of mice immunized with SWA or SEA,or the splenocytes of normal mice were harvested and stimulated with SWA or SEA in vitro.Then,the proportions of IFN γ and IL 4 producing cells in splenocytes,and the proportions of Th1 and Th2 cells in CD4 + T cells were determined by FACS,respectively.Results Compared to the SWA stimulation group,the higher proportions of IL 4 producing cells in splenocytes and of Th1 cells in CD4 + T cells were observed un der the SEA stimulation group(P < 0.05).Whereas SWA induced the significantly higher proportions of IFN γ producing cells in splenocytes and of Th2 cells in CD4 + T cells than those in the SEA stimulation group(P < 0.05).Conclusion The significantly higher levels of Th1 cells are only observed under SWA induction,however,the differentiation of Th2 cells in response to SEA stimulation is significantly more than that in response to SWA stimulation.更多还原