【摘要】 通过在人工饲料中添加不同浓度Zn2+(75,150,300,600,1 200 mg/kg),研究了饲料中不同浓度Zn2+在连续2代不同发育阶段黑水虻体内的积累及幼虫不同组织的分布规律.结果表明,过量的Zn2+可在6龄幼虫、蛹和成虫体内积累,且Zn2+含量为6龄幼虫>成虫>蛹,然而,同一世代不同发育阶段虫体内的Zn2+含量不存在显著差异;但第2代受高剂量Zn2+胁迫虫体内Zn2+含量均显著高于第1代.Zn2+可在黑水虻5龄末幼虫表皮、脂肪体及中肠中累积,连续2代分布规律均为中肠>脂肪体>表皮,第2代各组织中Zn2+含量均高于第1代;在300 mg/kg Zn2+浓度下,第2代5龄末幼虫表皮和脂肪体中的Zn2+浓度与第1代存在显著差异,在高剂量(600和1 200 mg/kg)Zn2+处理浓度下,中肠中Zn2+含量显著高于第1代.更多还原

【Abstract】 Artificial diets added with different concentrations of Zn2+(75,150,300,600,1 200 mg/kg) were fed on black soldier fly Hermetia illucens L.larvae for 2 generations,and contents of Zn2+ accumulation in different developmental stages of H.illucens and distribution in different tissues of the 5th instar larvae were detected.Results showed that excessive Zn2+ accumulate in the 6th instar larvae,pupae and adults,and the order of Zn2+ contents in different stages is 6 instar larvae > adults > pupae.However,no significant difference is occurred in the different developmental stages’ H.illucens from the same generation.At the same time,Zn2+contents from ones stressed with high-levels of Zn2+ in the 2nd generation are significantly higher than those in the 1st generation.Zn2+ accumulate in epidermis,fat body and midgut of the 5th instar larvae.In the 2 successive generations,Zn2+ content in different tissues is in the order of midgut > fat body > epidermis,and the Zn2+ contents in 2nd generation are all significantly higher than those in the 1st generation.Furthermore,Zn2+ contents in epidermis and fat body of the 2nd generation are significantly higher than those in the 1st generation under treatment with 300 mg/kg Zn2+.Significantly higher Zn2+ contents are found in midgut of 600 and 1 200 mg/kg Zn2+ stressed larvae from the 2nd generation when comparing to the 1st generation.更多还原