【摘要】 本研究旨在了解猪源新型甲型H1N1流感病毒山东分离株的遗传进化特点。对山东地区出现的疑似H1N1流感病死猪进行病料采样,然后进行病毒的分离鉴定,并对分离病毒株(A/swine/Shandong/07/2011)的HA、NA、PB2、PB1、PA、NP、NS和M基因进行遗传进化分析。结果显示,该株病毒8个片段的核酸序列与A/H1N1(2009)对应序列的相似性都大于99%,并且该毒株HA蛋白的裂解位点和优先识别唾液酸α-2,6受体的位点与A/H1N1(2009)也高度一致,分别为PSIQSR↓GLFGAI和190D、225D。但是,与A/H1N1(2009)毒株的HA蛋白相比,受体结合位点处出现了重要的突变(Q226R)。该研究结果为进一步研究猪源新型甲型H1N1流感病毒的分子进化提供了重要信息。更多还原

【Abstract】 The study was carried out to examine the genetic characteristics of a novel swine-origin influenza A(H1N1) virus isolated from Shandong province.Samples from suspected influenza-infected pigs were collected for viral isolation and identification,and then genetic evolution of HA,NA,PB2,PB1,PA,NP,NS and M of the isolate(A/swine/Shandong/07/2011) was analyzed and compared with the related influenza viruses.The results showed that sequences of 8 fragments of the isolated virus revealed >99% nucleotide identity with A/H1N1(2009) prototype strain,and its HA cleavage and receptor-binding sites were PSIQSR↓GLFGAI and 190D,225 D,respectively,which were identifical with A/H1N1(2009).However,compared with HA protein of A/H1N1(2009),mutation Q226R occurred in receptor binding site of the isolate.In conclusion,the results of this study provided significant information for further research on molecular evolution of novel swine-origin influenza A(H1N1) virus.更多还原