【摘要】 目的:探讨PCR+膜杂交法CT/UU/NG三联检测在临床性病筛查中的应用价值。方法:分别采用PCR+膜杂交法和实时荧光PCR法检测CT、UU、NG,并分析比较两种方法的一致性以及相关性。结果:PCR反向膜杂交法和实时荧光PCR法的CT、UU、NG的筛查结果接近(P>0.05),而男女筛查结果差异较大(P<0.05)。结论:PCR反向膜杂交法操作简便,筛查快速、准确,有与实时荧光PCR法相当的检测准确性,具有较高的临床应用价值,值得在临床中推广应用。更多还原

【Abstract】 Objectives: To study the clinical application value of PCR + membrane hybridization method CT/UU/NG detection in STD screening.Methods: We use PCR + membrane hybridization method to detect CT,UU and NG.We also use real-time fluorescent quantitative PCR to detect CT,UU and NG.We compare the results of the two approaches.Results: The results of both methods showed no significant difference(P>0.05).Men’s screening results were significantly different from those of women(P<0.05).Conclusion: PCR membrane hybridization is a simple and rapid method which is as accurate as real-time fluorescent PCR.It is of high value for clinical application.更多还原