【摘要】 目的探讨非酒精性脂肪肝脂联素基因SNP+45、SNP+276单核苷酸多态性与非酒精性脂肪肝(NAFLD)相关指标以及血清脂联素(APN)水平的相关关系。方法从2011年新疆克拉玛依地区非酒精性脂肪肝流行病学调查中随机选出符合NAFLD纳入标准的NAFLD组255例,健康对照组405例。采用聚合酶链限制性片段长度多态(PCR-RFLP)方法鉴定APN基因SNP+45、SNP+276核苷酸多态性。结果SNP45G、SNP276G位点的Age、BMI、WHR、SBP、DBP、FBG、AST、TG、CHO、APN、SUA偏高,HDL偏低。SNP45G携带者NAFLD患病率高于TT携带者(41.1%>36.9%),差异无统计学意义(P>0.05);SNP276T突变基因携带者NAFLD患病率低于GG携带者(32.8%<43.5%),差异具有统计学意义(P<0.05)。NAFLD组和对照组人群中SNP+45多态性位点分布为:TT型50.4%和55.3%,GT型43.1和41.5%,GG型6.7%和3.2%;SNP+276多态性位点分布为:TT型9.8%和12.6%,GT型29.4%和37.8%,GG型60.8%和49.6%。两组SNP+45多态性位点基因型频率及等位基因频率的分布差异无统计学意义(P>0.05);NAFLD组SNP+276出现突变基因型的频率明显低于对照组,差异均有统计学意义(P<0.05)。Logistic回归分析显示,APN276T等位基因、血清脂联素水平是NAFLD发病的独立保护因素;Age、WC、BMI是NAFLD发病的独立危险因素,而APN45T>G基因多态性与非酒精性脂肪性肝病的发病无相关关系(P>0.05)。结论 APN+276G/T多态性与NAFLD的发生有关,APN276T突变等位基因是NAFLD的独立保护因素;未发现APN+45多态性与NAFLD的发病有关。APN+45、+276多态性影响血清脂联素的表达,携带非突变的45T与突变的276T等位基因的个体血清脂联素水平高。更多还原

【Abstract】 Objective To investigate the relationship among the adiponectin gene polymorphisms,plasma levels of adiponectin,and risk of nonalcoholic fatty liver disease(NAFLD).Methods 255subjects of NAFLD(case group)were selected from epidemiological investigation in Karamay health check up center in 2011.405normal subjects without NAFLD were selected as control group.The APN45T>G,276G> T polymorphisms were determined by polymerase chain reaction-restriction fragment length polymorphism(PCR-RFLP).Results Age,BMI,WHR,SBP,DBP,FBG,AST,TG,CHO,APN,SUA in subjects with SNP45Gand SNP276Gwere higher,HDL was Lower.The prevalence of SNP45Gcarriers are significantly higher than that of TT carriers(41.1%>36.9%),no significant difference(P >0.05);SNP276T mutation carriers of NAFLD prevalence rate was lower than the GG carriers(32.8% < 43.5%),the difference was statistically significant(P <0.05).The distributions of genotypes and alleles of APN SNP45+in the cases/the controls were as follows:TT 50.4%/55.3%,GT 43.1%/41.5%,GG 6.7%/ 3.2%.The distributions of genotypes and alleles of APN +SNP276+in the case/the controls were as follows:TT 9.8%/12.6%,GT 29.4%/37.8%,GG 60.8%/49.6%.Significant difference in genotypes and alleles of SNP+45between two groups was found(P>0.05).NAFLD group of SNP+276mutation genotype frequency was significantly lower than that of the control group,the difference was statistically significant(P <0.05).Logistic regression showed that serum adiponectin levels,APN SNP276Tallele were all independent protective factors for NAFLD,while age,BMI and Waist was all independent risk factor for NAFLD.No correlation between APN45T>G gene polymorphisms and the pathogenesis of nonalcoholic fatty liver disease(P >0.05).Conclusion The adiponectin gene 276G>T polymorphism is significantly associated with the presence of NAFLD and the allele of adiponectin gene 276Tis independent protective factor for the incidence of NAFLD.Not found APN+45polymorphism and the pathogenesis of NAFLD.The adiponectin gene 276G/T,45T/G polymorphism has an effect on expression of serum adiponectin levels and subjects carrying the 276T,45Tallele have higher serum adiponectin levels.更多还原