【摘要】 目的建立高效液相法测定胡芦巴提取物含量的方法,研究不同胡芦巴提取物对DPPH及羟基自由基(.OH)的清除效果。方法采用药典方法测定新疆、安徽产胡芦巴、胡芦巴醇提物及胡芦巴总皂苷(TFGs)中的胡芦巴碱;薯蓣皂苷元的含量测定,色谱柱为大连伊力特Hypersil ODS-1(4.6mm×250mm,5μm);流动相为甲醇-水(86∶14,v/v),流速1.0mL/min,检测波长203nm,柱温35℃;紫外法测定体外抗氧化作用。结果薯蓣皂苷元的色谱峰面积与浓度呈良好的线性关系,线性范围为43.2～864μg/mL(r=0.999 5);平均回收率(n=9)为100.3%。胡芦巴碱含量:胡芦巴(新疆)0.66%、胡芦巴(安徽)0.62%,胡芦巴醇提物1.78%、胡芦巴大孔树脂提取物(TFGs)0.47%;薯蓣皂苷元含量:胡芦巴(新疆)0.78%、胡芦巴(安徽)0.85%,胡芦巴醇提物4.94%、胡芦巴大孔树脂提取物(TFGs)7.58%;胡芦巴各提取物对DPPH的清除IC50值:TFGs为1.2mg/mL、胡芦巴醇提物为0.6mg/mL、胡芦巴碱提取物为5.0mg/mL;胡芦巴各提取物对羟自由基的清除IC50值:TFGs为6.5mg/mL、胡芦巴醇提物为3.3mg/mL、胡芦巴碱提取物为12.5mg/mL。结论采用HPLC法测定胡芦巴提取物中薯蓣皂苷元的含量,方法准确可靠、重现性好,可用于含量测定;胡芦巴各提取物具有清除DPPH和羟基自由基的作用。更多还原

【Abstract】 Objective To establish a method for assaying the extracts of fenugreek with the support of high pressure liquid chromatography(HPLC)and explore the scavenging activity of different extracts on DPPH and hydroxyl freebase(.OH).Methods The fenugreeks grown in Xinjiang and Anhui,ethanol extracts of fenugreeks and the trigonelline in TFGs were detected with the adoption of CHP method.The diosgenin was analyzed by HPLC on Hypersil ODS-1columns(4.6mm×250mm,5μm)with the mobile phase of methanol acetonitrile-water(86∶14,v/v).Its flow rate was 1.0mL/min,the detection wavelength was 203nm,and the column temperature was 35℃.The antioxidation activity in vitro was examined by UV method.Results The linearity range of diosgenin was 43.2-864μg/mL(r=0.999 5)and its average recovery rate(n=9)was100.3%,which presented a good linear relationship between the chromatographic peak area of the diosgenin and its concentration.The contents of trigonelline:0.66% fenugreek(Xinjiang),0.62%fenugreek(Anhui),1.78% ethanol extracts of fenugreek,0.47% macroporous rasin extracts.The contents of diosgenin:0.78%fenugreek(Xinjiang),0.85%fenugreek(Anhui),4.94%ethanol extracts of fenugreek,7.58% macroporous rasin extracts;The IC50scavenging value of each fenugreek extract on DPPH:TFGs,1.2mg/mL;ethanol extracts of fenugreek,0.6mg/mL;alkaloid extracts of fenugreek,5.0mg/mL.The IC50scavenging value of each fenugreek extract on hydroxyl freebase:TFGs,6.5mg/mL;ethanol extracts of fenugreek,3.3mg/mL;alkaloid extracts of fenugreek,12.5mg/mL.Conclusion The content of diosgenin in fenugreek extracts was detected by HPLC method.HPLC method is accurate,reliable and of good reproducibility;It could be adopted in the quantitative determination of content;Fenugreek extracts have scavenging effects on DPPH and hydroxyl freebase.更多还原